两种桃芽总RNA提取方法的比较研究  

COMPARISON STUDY ON TWO MODIFIED EXTRACTING METHODS FOR PEACH BUDS TOTAL RNA

作　　者：王宇[1] 李玲[1] 王慧[1] 陈修德[1] 高东升[1] 

机构地区：[1]山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,山东泰安271018

出　　处：《山东农业大学学报（自然科学版）》2011年第3期388-391,共4页Journal of Shandong Agricultural University：Natural Science Edition

摘　　要：以‘曙光油桃’芽为试材,采用改良CTAB法、Trizol法进行总RNA提取,并通过凝胶电泳、核酸蛋白分析仪和Q-PCR检测所提取总RNA样品的质量。结果表明:改良CTAB法提取的RNA,经Q-PCR后扩增桃芽HK基因,可以得到与目的片段大小一致的条带,条带清晰,能满足基因表达分析等后续试验的要求;而Trizol法所提取的RNA,条带较弱,经Q-PCR后扩增桃树芽内HK基因,得不到与目的片段大小一致的条带,难以达到进一步试验的要求。The bud of peach（Prunus persica var.nectariana cv.Shuguang） was used as material for extracting total RNA with Modified CTAB method and Trizol extraction kit.The quality of the total RNA was tested by gel electrophoresis,nuclear acid/protein analyzer and Q-PCR.The results showed that the RNA got from CTAB method had a better quality with a distinct bands,and using this RNA to amplify HK gene in peach bud by Q-PCR can obtain the target fragments,and could satisfy the requirement of molecular biology trial.However,the RNA got from Trizol extraction kit had weak bands,and cannot get the target fragments of HK gene in peach bud by Q-PCR,thus it cannot meet the requirements of further tests.

关 键 词：曙光油桃 芽 总RNA 提取 

分 类 号：Q522[生物学—生物化学]