花生种子不同部位诱导愈伤和丛生芽的条件初探  

Preliminary report on the in vitro culture of somatic mebryo and adventitious shoots induction from different parts of peanut(Arachis hypogaea L.)seeds

作　　者：温世杰[1] 郑燕华[2] 刘海燕[1] 罗虹[2] 

机构地区：[1]广东省农科院作物研究所,广东广州510640 [2]华南师范大学生命科学院,广东广州510631

出　　处：《广东农业科学》2011年第16期13-15,共3页Guangdong Agricultural Sciences

基　　金：现代农业产业技术体系建设专项;粤港关键领域重点突破项目(200849863)

摘　　要：以花生种子为试验材料,对花生种子的不同部位用不同激素浓度组合进行诱导,探讨花生体细胞胚和丛生芽的诱导效果。结果表明,完整胚在MS+0.1 mg/L TDZ(ThidjazUron)培养基上分化丛生芽的频率高达100%;在MS+0.1 mg/L TDZ+3 mg/LPIC(Picloram)培养基上的出愈率达98%,达到了提高繁殖系数和遗传转化基础的要求。For improving peanut（Arachis hypogaea L.） propagation coefficient and the basic requirements of genetic transformation,to induce somatic mebryo and adventitious shoots using the different parts of peanut（Arachis hypogaea L.）seeds in different hormone concentration.The results showed that the adventitious shoots were induced from the complete embryo cultured on MS medium supplemented with 0.1 mg/L TDZ,the frequencies was obtained in 100%;and on MS medium supplemented with 0.1 mg/L TDZ and 3 mg/L PIC,the frequencies was obtained in 98%.

关 键 词：花生 噻苯隆 毒莠定 体细胞胚 丛生芽 

分 类 号：S565.203.53[农业科学—作物学]