寄生线虫真菌黑葡萄穗霉的分离与鉴定  

Isolation and identification of a parasitic-nematode fungal strain:Stachybotrys chartarum

作　　者：李玉中[1,2] 李本祥[1] 王彬[3] 卓侃[1] 廖金铃[1] 

机构地区：[1]华南农业大学资源环境学院,广东广州510642 [2]衡阳师范学院生命科学系,湖南衡阳421008 [3]徐州空军学院航空军需系,江苏徐州221000

出　　处：《广东农业科学》2011年第16期59-61,共3页Guangdong Agricultural Sciences

基　　金：国家公益性行业(农业)科研专项(nyhyzx07-050-4;200903040);广东省科技攻关重点项目(2005B20801013);衡阳师范学院科技基金(08B05)

摘　　要：利用水琼脂平板撒土——腐生线虫诱导法从根结线虫危害的作物根际土壤分离到一株线虫寄生真菌。PDA平板上形态观察显示,菌落黑色凸起,气生菌丝较多,分生孢子梗伞状,产孢结构为瓶状小梗,分生孢子椭圆或近圆形,未成熟孢子光滑,深棕色到黑色,成熟时黑色有棘。经分子鉴定,该菌为黑葡萄穗霉(Stachybotrys chartarum)。该菌在水琼脂平板上可生长,菌丝稀疏,可侵染并寄生腐生线虫Panagrellus redivius.The strain of S.chartarum was isolated from the soil,was taken from rhizospheres of crops that infected by root-knot nematodes,by the method of scattering soil on the water agar plate and saprophytic nematode-induced.On PDA,colony of the fungus was black with more aerial hyphae.Its conidiophores were umbrella with 4~6 bottle-shaped conidiogenous structure at the distal end.Both smooth immature spores and mature spores with spines were oval or nearly round,dark brown to black.In the water agar plate,the sparse mycelium can infect and parasite Panagrellus redivius.

关 键 词：黑葡萄穗霉 寄生 线虫 生物防治 

分 类 号：S476.15[农业科学—农业昆虫与害虫防治]