冬凌草抗菌霜剂的制备和局部抗感染实验  

Preparation and Anti-infective Activity of Rabdosia Rubescens Topical Cream

作　　者：梁菊[1] 陈姗[1] 刘秋伟[1] 

机构地区：[1]河南科技大学化工与制药学院,河南洛阳471003

出　　处：《河南科技大学学报（自然科学版）》2011年第5期101-104,10,共4页Journal of Henan University of Science And Technology:Natural Science

基　　金：河南省科技发展计划基金项目(092102210182);河南省教育厅自然科学研究计划项目(2011A350001);河南科技大学基金项目(2010)

摘　　要：以济源产冬凌草的醇提浸膏为主成分,制备了一种皮肤外用霜剂,对其安全性和局部皮肤抗感染作用进行了实验研究。乙醇回流法获得冬凌草的醇提物浸膏,体外抑菌实验结果显示:该提取物对枯草芽孢杆菌、金黄色葡萄球菌和苏云金杆菌具有明显的抑制活性。以该提取物浸膏为主成分,制作了一种皮肤外用霜剂。急性皮肤刺激性实验结果表明:该霜剂对正常皮肤及破损皮肤均无刺激性。该霜剂对金黄色葡萄球菌引起的局部皮肤感染有明显的治疗效果,每天给药3次,每次给药0.2 g,7d后皮肤红肿现象完全消失。局部皮肤体外微生物培养实验发现:注射部位皮肤已无病原菌感染。该研究为临床开发冬凌草的外用制剂提供重要的实验依据。In this paper,a topical skin cream containing ethanol extract of Rabdosia rubescens from Jiyuan of Henan province was prepared.The safety and anti-infective activity of this cream were evaluated.The ethanol extract from Rabdosia rubescens was obtained by ethanol refluxing method.Results of antibacterial experiment showed the obvious bacteriostasis on Bacillus subtilisin,Staphylococcus aureus and Bacillus thuringiensis in a concentration dependent manner.A topical cream was formulated with ethanol extract as the main ingredient and was evaluated for acute skin irritancy in which the cream showed less irritant and safe for topical application.The guinea pig model of Staphylococcus aureus intradermal infection was then established and used for testing anti-infective activity of the cream.Skin redness and swelling disappeared after administered 7 days with 0.2 g of the cream three times a day.And the injected area had no bacteria survival by the bacterial culture of local skin in media.Those results showed that Rabdosia rubescens topical cream might be developed for potential clinical anti-infection drug.

关 键 词：冬凌草 醇提物 霜剂 抗感染 

分 类 号：R944.9[医药卫生—药剂学]