阉割后草原红牛与正常公牛8种组织中IGFⅠ和IGFⅡ基因表达差异的研究  

The Research of the IGFⅠand IGFⅡ Expression Differences Between Castrated Chinese Red Steppe and Normal Bull in Eight Tissues

作　　者：孟纪伦[1] 赵志辉[1] 秦立红[1] 张国梁[2] 赵玉民[2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林省农业科学院畜牧分院

出　　处：《青岛农业大学学报（自然科学版）》2011年第2期121-125,共5页Journal of Qingdao Agricultural University(Natural Science)

基　　金："十一五"国家高技术研究计划(863计划)(2008AA101010);国家支撑计划(2007BAD55B01);转基因专项(2009ZX08007-005B;2009ZX08009-156B;2008ZX08007-001)

摘　　要：本研究采用实时荧光定量RT-PCR技术检测了胰岛素样生长因子1(IGFⅠ)和胰岛素样生长因子2(IGFⅡ)基因在18月龄草原红牛阉割后牛与公牛心脏、肝脏、脾脏、肺脏1、肾脏、瘤胃、十二指肠、背最长肌组织等8种组织中的mRNA表达水平。旨在探讨该基因在阉割后草原红牛与正常公牛不同组织中的表达差异。结果表明:IGFⅠ和IGFⅡ基因在所检测的阉割后牛与正常公牛的心脏、肝脏、脾脏、肺脏、肾脏、瘤胃、十二指肠、背最长肌组织等8种组织中均有表达。且①IGFⅠ在肝脏和瘤胃中的表达存在差异,阉割后牛在肝脏中IGFⅠ基因的表达水平显著高于公牛(P<0.05),但是在十二指肠中则显著低于正常公牛(P<0.05);②阉割后牛IGFⅡ基因在心脏和肺脏的表达水平显著高于正常公牛(P<0.05)。本研究结果初步揭示了阉割后草原红牛与正常公牛IGFⅠ和IGFⅡ基因表达的差异,为深入研究IGFⅠ和IGFⅡ基因的生物学功能及了解这两个基因对阉割个体肉质性状的形成机理提供了基础数据。The present study was designed to detect the expression of IGF Ⅰ and IGF H mRNA in the heart, liver, spleen, lung, kidney, rumen, duodenum, longissimus dorsi of steer and normal bull Chinese Red Steppe by Real - time RT - PCR. The results showed that IGF I and IGF Ⅱ mRNA was expressed in all of eight tissues ： heart, liver, spleen, lung, kidney, rumen, duodenum, longissimus dorsi of bull and steer. We also found that ① IGF Ⅰ gene expression level has difference in liver and rumen. IGF I mRNA expression level was significant higher in liver of steer than bull（ P 〈 0.05 ） ; but the expression level of ICF Ⅰ mRNA was significant lower in duodenum of steer than bull（ P 〈0.05 ）. ② IGF H mRNA expression level was significant higher in heart and lung of steer than bull （ P 〈 0.05 ）. The research showed preliminary pattern of the expression of IGF Ⅰ and IGF Ⅱ mRNA in steer and bull, it ,provides the basis for the future studies of the biological function and understanding the mechanisms oflGF Ⅰ and IGF H gene.

关 键 词：中国草原红牛 实时荧光定量PCR 胰岛素样生长因子 

分 类 号：S813[农业科学—畜牧学]