斑点叉尾鮰病毒多克隆抗体的制备及其生物活性鉴定  

Preparation and bioactivity of polyclonal antibody against channel catfish virus

作　　者：吴兵[1] 罗宇良[1] 王敏[1] 曹胜波[2] 王卫民[1] 陈龙[2] 黄锋涛[1] 刘学芹[1] 

机构地区：[1]华中农业大学水产学院/农业动物遗传育种与繁殖教育部重点实验室,武汉430070 [2]华中农业大学动物医学院,武汉430070

出　　处：《华中农业大学学报》2011年第5期631-634,共4页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30901118);教育部新教师基金项目(20070504047);湖北省自然科学基金项目(2008CBB099)

摘　　要：利用斑点叉尾鮰卵巢细胞增殖斑点叉尾鮰病毒,然后将经过超速离心纯化的病毒免疫4周龄BABL/C雌鼠。每2周免疫1次,第4次免疫2周后,经眼眶采血,分离血清,通过酶联免疫吸附试验检测其抗体效价,并利用间接免疫荧光试验和与病毒孵育试验检测所制备多克隆抗体的生物活性。结果表明,制备的多克隆抗体ELISA效价为1∶25 600,间接免疫荧光试验证明该多克隆抗体特异性良好,同时与病毒的孵育试验证明该多克隆抗体具备中和活性,能够有效阻断斑点叉尾鮰病毒对细胞的感染。Channel catfish virus was propagated in the channel catfish ovary cell line and then purified by ultracentrifugation.This purified virus was used to immunize the female BALB/C mice to gain polyclonal antibody.ELISA was used to detect the antibody titer.The bioactivity was identified by indirect immunofluorescent assay and serum neutralization tests.The ELISA titer of the polyclonal antibody was 1∶25 600.The polyclonal antibody showed high specificity at the indirect immunofluorescent assay.It could stop the virus from infecting the cell efficiently.

关 键 词：斑点叉尾鮰病毒 多克隆抗体 制备 效价 生物活性 

分 类 号：S941[农业科学—水产养殖]