活体肝细胞凋亡代谢改变的质子磁共振频谱研究  

Metabolic changes of liver cell apoptosis:an in vivo 1H magnetic resonance spectroscopy study

作　　者：尤克增[1] 沈智威[1] 曹震[1] 杨忠现[1] 成小芳[1] 姚建莉[1] 吴仁华[1] 

机构地区：[1]汕头大学医学院第二附属医院影像科,汕头515041

出　　处：《磁共振成像》2011年第4期290-295,共6页Chinese Journal of Magnetic Resonance Imaging

基　　金：广东省医学科研基金(B2008128);国家自然基金(30930027;60971075)资助

摘　　要：目的探索1H-MRS检测活体肝脏损伤中肝细胞凋亡的可行性,并寻找频谱的特征性改变。方法新西兰大白兔随机分成对照组(10只)和实验组(17只),分别腹腔注射生理盐水和亚硒酸钠48h后,使用单体素PRESS序列扫描活体肝脏。在MRS扫描后,立即牺牲动物,并取肝组织进行病理学检查。磁共振频谱数据通过SAGE7.0软件进行处理,分析未抑水频谱的脂质/水(Lip/Water)和胆碱复合物/水(tCho/Water)和抑水频谱的胆碱复合物/脂质(tCho/Lip)的面积比。使用双侧独立t检验进行统计分析。结果 25只动物最终完成该实验过程(实验组15只,对照组10只)。对基线平稳并且代谢物峰位置正确的1H-MRS谱线波峰进行分析。实验组和对照组活体肝脏磁共振频谱有差异。对未抑水频谱,实验组Lip/Water(0.029±0.024,n=12)比对照组该指标(0.014±0.007,n=10)相对升高;实验组tCho/Water(0.001±0.0005,n=12)较对照组该指标(0.0030±0.0003)降低,上述两组数据均具有统计学差异(P<0.05)。实验组的tCho/Lip(0.093±0.007,n=12)较对照组该指标(0.210±0.061,n=10)降低,具有统计学差异(P<0.05);实验组凋亡细胞百分数为(9.2±3.1)%,对照组凋亡细胞占(0.27±0.07)%,两组间存在显著差异(P<0.001)。结论实验组和对照组活体肝脏磁共振频谱有差异,肝脏脂质(Lip)和胆碱复合物(tCho)具有一定特征性变化,活体1.5T磁共振频谱可用于检测肝脏损伤和细胞凋亡的代谢改变。Objective：To explore the feasibility of dectecting liver cell apoptosis in liver injury model by using magnetic resonance spectroscopy（MRS）,and endeavor to find the characteristic changes of MRS.Materials and Methods：Twenty-seven New Zealand white rabbits were randomly divided into the control group（n=10） and the treated group（n=17）.The rabbits in the treated group were intraperitoneally（i.p.） injected with sodium selenite while the ones in the control group with saline.At 48 hours after the injection,in vivo liver 1H-MRS were collected using point resolved spectroscopy sequence under the same conditions.After MR scanning,the rabbits were sacrificed immediately for histopathological examination.The 1H-MRS data were processed by SAGE 7.0 software to analyze the ratio of lipid/water（Lip/Water） and total choline/water（tCho/Water） in unsuppressed water spectral and the ratio of total choline/lipid（tCho/Lip） in suppressed water spectra.Statistical analysis was done by a two-tailed independent t test.Results：All of the listed tests were fully finished in 25 rabbits（15 treated and 10 controls）.The MRS curves,mainly with peaks appearing at correct positions,could be analyzed.From MR spectra,there are some differences between the treated group and the control one.In unsuppressed water spectra,the ratio of Lip/Water（0.029±0.024,n=12） in treated group is higher than that in control group（0.014±0.007,n=10）（P0.05）;the ratio of tCho/Water（0.001±0.0005,n=12） in the treated group is significantly lower than that（0.0030±0.0003） in control group（P0.05）.In suppressed water spectra,the ratio of tCho/Lip（0.093±0.007,n=12） in the treated group is also significantly lower than that in control group（0.210±0.061,n=10）（P0.05）.The percentage of apoptotic cells was 9.2±3.1% in the model rats,and 0.27±0.07% in controls（P0.001）.Conclusion：After the injections,there are some characteristic differences between the 1H-MRS in the treatment group and that in th

关 键 词：细胞凋亡 磁共振频谱 肝脏 

分 类 号：R445.2[医药卫生—影像医学与核医学] R256.4[医药卫生—诊断学]