榄香烯对HLE-B3内蛋白激酶A及其mRNA表达的影响  

Effect of Elemene on Expression Levels of Protein Kinase A and Its mRNA in Human Lens Epithelial Cell B3

作　　者：胡艳红[1] 黄秀榕[2] 祁明信[1] 侯补元[2] 

机构地区：[1]福建中医药大学附属第二人民医院,福建福州350003 [2]福建中医药大学病理生理研究中心,福建福州350003

出　　处：《福建中医药大学学报》2011年第4期16-18,共3页Journal of Fujian College of Traditional Chinese Medicine

基　　金：福建省科技厅青年人才基金项目(2008F3050)

摘　　要：目的探讨中药单体榄香烯(Ele)对人晶状体上皮细胞系(HLE-B3)内蛋白激酶A(PKA)及mRNA表达的影响。方法利用碱性成纤维细胞生长因子(rhbFGF)诱导HLE-B3增殖,将80mg/L的Ele作用在处于增殖状态下的HLE-B3 24 h,流式细胞术检测HLE-B3内PKA的表达,RT-PCR法检测HLE-B3内PKA mRNA表达。结果 rhbFGF组HLE-B3内PKA表达(33.250±2.242)较空白组(46.847±1.673)明显下降(P<0.01),Ele组(64.110±2.933)较rhbFGF组明显升高(P<0.01)。rhbFGF组HLE-B3内PKA mRNA表达(0.734±0.041)较空白组(0.927±0.040)明显下降(P<0.01),Ele组(1.196±0.066)较rhbFGF组明显升高(P<0.01)。结论上调HLE-B3内PKA表达、激活细胞内PKA信号转导途径可能是Ele抑制HLE-B3增殖的作用机制。Objective To investigate the effect of elemene（Ele） on expression levels of protein kinase A（PKA） and its mRNA in human lens epithelial cells B3（HLE-B3）.Methods The proliferation of HLE-B3 was induced by 10 ng/mL recombinant human basic fibroblast growth factor（rhbFGF）,then proliferative HLE-B3 were incubated with 80 mg/L Ele in CO2 incubator for 24 hours.The expression levels of PKA and its mRNA in HLE-B3 were detected by flow cytometry,RT-PCR respectively.Results Compared with control group,the expression level of PKA,PKA mRNA in HLE-B3 in rhbFGF group was decreased respectively（P0.01）.Compared with rhbFGF group,the expression level of PKA,PKA mRNA in HLE-B3 in Ele group was increased respectively（P0.01）.Conclusion Ele could up-regulate PKA and its mRNA expressions and activate PKA signal transduction pathway in HLE-B3,which was probably the mechanism of proliferative inhibition of HLE-B3 by Ele.

关 键 词：榄香烯 晶状体上皮细胞 后发性白内障 信号转导 

分 类 号：R285.5[医药卫生—中药学]