小鼠皮肤切创愈合过程中纤维细胞募集和分化的时间依赖性  

Time-dependent Recruitment and Differentiation of Fibrocytes in Mouse Skin Wound Healing

作　　者：范琰琰[1] 官大威[1] 王涛[1] 刘伟伟[1] 郑吉龙[1,2] 赵振宾[1] 于天水[1] 马文翔[1] 

机构地区：[1]中国医科大学法医学院,辽宁沈阳110001 [2]中国刑警学院法医学系,辽宁沈阳110854

出　　处：《法医学杂志》2011年第4期246-249,共4页Journal of Forensic Medicine

摘　　要：目的观察小鼠皮肤切创愈合过程中,纤维细胞募集和分化随时间变化的规律性。方法应用免疫荧光技术共定位纤维细胞[共表达CD45、Ⅰ型前胶原(procollagenⅠ)]和肌成纤维细胞[共表达CD45、平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)],计数纤维细胞及其分化成的肌成纤维细胞。结果纤维细胞于切创后3d募集于创口局部,其分化成的肌成纤维细胞于切创后5d开始出现于肉芽区。纤维细胞及其分化成的肌成纤维细胞于伤后7d达到峰值。结论在小鼠皮肤切创愈合过程中,肉芽组织中的肌成纤维细胞至少部分来源于纤维细胞的分化,纤维细胞时间依赖性地募集和分化可以为损伤时间的推断提供新的信息。Objective To investigate the time-dependent recruitment and differentiation of fibrocytes in skin wound healing. Methods Fibrocytes （expressing CD45 and proeollagen [ ） and myofibroblasts （expressing CD45 and ol-SMA） were co-localized by immunofluorescent staining. The number of fibrocytes and myofi- broblasts was counted at different post-wounding interval. Results At 3 d after injury, fibrocytes started to recruit at the margin of the wounds. At 5 d after injury, myofibroblasts started to appear in new formed granulation tissue. The number of fibrocytes and myofibroblasts peaked at 7 d post-wounding. Conclusion During skin wound healing, myofibroblasts in granulation tissue originated at least partly from fibrocytic differentiation. The time-dependent recruitment and differentiation of fibroeytes may provide new informa- tion for wound age determination.

关 键 词：法医病理学 创伤和损伤 皮肤 纤维细胞 肌成纤维细胞 损伤时间 小鼠 

分 类 号：DF795.1[医药卫生—法医学]