大鼠弥漫性轴索损伤后海马热休克蛋白质70 mRNA表达  

Expression of HSP70 mRNA in Hippocampus of Rats after Diffuse Axonal Injury

作　　者：汪枫[1] 李永宏[1] 

机构地区：[1]皖南医学院法医学系

出　　处：《法医学杂志》2011年第4期250-252,共3页Journal of Forensic Medicine

摘　　要：目的研究大鼠弥漫性轴索损伤(diffuse axonal injury,DAI)后大脑海马中热休克蛋白质(heat-shockproteins,HSP)70 mRNA的表达变化。方法采用RT-PCR扩增,扩增产物用琼脂糖凝胶电泳检验,观察DAI后大鼠海马中HSP70 mRNA的表达变化。结果损伤后4 h,大鼠海马中HSP70 mRNA的表达增加,24 h表达达高峰,48 h后表达减弱。结论大鼠海马中HSP70 mRNA表达可作为DAI诊断的参考指标。Objective To explore the changes of expression of heat-shock proteins （HSP） 70 mRNA in hippocampus of rats after diffuse axonal injury （DAI）. Methods RT-PCR products of HSP70 mRNA were tested by agarose gel electrophoresis after RT-PCR amplification. The changes of HSPT0 mRNA expression were observed in rat hippocampus after DAI. Results The expression of HSP70 mRNA in the hippoeampus could be detected 4 h after DAI. It reached maximum at 24 h and declined after 48 h. Conclusion HSP70 mRNA in hippocampus of rats could be useful for diagnosis of DAI

关 键 词：法医病理学 弥漫性轴索损伤 海马 HSP70热休克蛋白质类:大鼠 

分 类 号：DF795.1[医药卫生—法医学]