GC-MS同时测定血液中苯海索、氯丙嗪和氯氮平  

Simultaneous Determination of Trihexyphenidyl,Chlorpromazine and Clozapine in Blood by GC-MS

作　　者：郑水庆[1] 王威[1] 梁晨[1] 汪蓉[1] 龚飞君 吴忠平[1] 陈永生[1] 张玉荣[1] 张润生[1] 

机构地区：[1]上海市公安局物证鉴定中心上海市现场物证重点实验室,上海200083

出　　处：《法医学杂志》2011年第4期271-273,共3页Journal of Forensic Medicine

摘　　要：目的建立GC-MS同时测定血液中苯海索、氯丙嗪和氯氮平的方法。方法血液中加入内标SKF525A,在pH>10条件下用V(苯)﹕V(乙酸乙酯)=1﹕1提取,用GC-MS全扫描法进行定性检测;以地西泮-d5为内标,样品在pH>10条件下用V(苯)∶V(乙酸乙酯)=1∶1提取,用GC-MS选择离子监测方法进行定量检测。结果苯海索、氯丙嗪和氯氮平在20～10000ng/mL范围内呈线性关系,最小检测限分别为0.3、0.3和0.7ng/mL(信噪比≥3),方法回收率为79.9%～85.5%,日内、日间精密度均小于5.1%。结论本方法可同时分析血液中苯海索、氯丙嗪和氯氮平,方法灵敏度高、快速、操作简便,适用于苯海索、氯丙嗪和氯氮平的血药浓度监测和急性中毒案件的检测。Objective To develop a method to measure trihexyphenidyl, chlorpromazine and clozapine in human blood with GC-MS. Methods The specimens were alkalized （pH〉10） and extracted with V（ben- zene）：V （ethyl acetate）= 1：1, and qualitatively analyzed using GC-MS-Full Scan with internal standard SKFs^A. The specimens were alkalized（pH〉10） and extracted with V（benzene）：V（ethyl acetate ） =l ： l, and quantita- tively analyzed using GC-MS-SIM with internal standard diazepam-ds. Results The lowest detection limits of trihexyphenidyl, chlorpromazine and clozapine were 0.3, 0.3 and 0.7ng/mL （S/N≥3） respectively. The calibration curve in 20-10 000 ng/mL showed a good linear distribution. The recovery rate was 79.9% to 85.5%. The RSDs of intraday and interday were less than 5.1%. Conclusion The established method was simple, sensitive and accurate for simultaneous determination of trihexyphenidyl, ehlorpromazine and elozap- ine in human blood, and can be applied in forensic toxicological cases.

关 键 词：法医毒理学 气相色谱-质谱法 苯海索 氯丙嗪 氯氮平 血液 

分 类 号：DF795.1[医药卫生—法医学]