RP-HPLC法测定雷公藤微乳中雷公藤内酯甲的含量  

Determination of Wilforlide in Tripterygium wilfordii multi-glucoside microe-mulsion by RP-HPLC

作　　者：王志君[1] 尤本明[1] 孙华君[1] 宋洪杰[1] 刘继勇[1] 

机构地区：[1]第二军医大学长海医院药学部,上海200433

出　　处：《药学实践杂志》2011年第4期269-271,共3页Journal of Pharmaceutical Practice

基　　金：国家自然科学基金(30801557);上海市科委中药现代化专项基金(09DZ1971102)

摘　　要：目的采用RP-HPLC法测定雷公藤微乳中雷公藤内酯甲的含量。方法液相色谱条件为:Hypersil C18柱(150 mm×4.6 mm,5μm),流动相为乙腈-水(90∶10);流速1.0 ml/min;柱温为室温;检测波长210 nm;进样量20μl。结果雷公藤内酯甲在2.0～30.0μg/ml范围内线性关系良好(r=0.999 9),平均回收率为(100.16±0.56)%(n=9),3批样品中雷公藤内酯甲的平均含量分别为标示量的(98.92±0.92)%。结论该方法准确、灵敏,专属性强,重现性好,可作为雷公藤微乳中雷公藤内酯甲的含量测定方法。Objective To develop a RP-HPLC method for the determination of Wilforlide in Tripterygium wilfordii multi-glu-coside microemulsion.Methods The separation was performed on a Kromasil ODS C18 analytical column（150 mm×4.6 mm,5μm）with the mobile phase consisting of acetonitrile-water（90∶10）.The detection wavelength was 210 nm.The column temperature was room temperature.The flow rate was 1.0 ml/min and the sample injection volume was 20 μl.Results Wilforlide presented a goodline relationship in the range of 2.0～30.0 μg/ml（r=0.999 9）.The average recovery rate was（100.16 ± 0.56） %（n=9）.The average contents of Wilforlide in three batch samples were（98.92 ± 0.92） %.Conclusion It was a sensitive,selective and reliable method for the determination of Wilforlide in Tripterygium wilfordii multi-glucoside microemulsion.

关 键 词：雷公藤 微乳 雷公藤内酯甲 含量测定 

分 类 号：R917[医药卫生—药物分析学]