秦艽与龙胆挥发油的化学成分及抗炎活性研究  

Composition and anti-inflammatory activities of essential oils from Gentiana crassicaulis and Gentiana scabra

作　　者：何希瑞[1,2] 李茂星[1] 尚小飞[1] 贾正平[1,2] 张汝学[1] 

机构地区：[1]兰州军区兰州总医院,甘肃兰州730050 [2]兰州大学,甘肃兰州730000

出　　处：《药学实践杂志》2011年第4期274-277,283,共5页Journal of Pharmaceutical Practice

基　　金：全军中医药研发推广项目(2006032001);甘肃省科技攻关项目(2GS054-A43-014-14)

摘　　要：目的研究秦艽和龙胆挥发油的化学成分和体内外抗炎活性。方法超临界CO2萃取秦艽和龙胆地下部分的挥发油;采用气相色谱-质谱联用方法分析萃取物的成分;采用LPS刺激小鼠巨噬细胞RAW264.7细胞炎症和二甲苯致小鼠耳肿胀试验,观察所得挥发油体内外抗炎活性。结果所得挥发油透明、橙黄、有浓郁的香味。通过GC-MS分析,从秦艽和龙胆挥发油中分别鉴定出29个和40个化合物。药理实验表明,秦艽和龙胆挥发油均能显著抑制由脂多糖诱导的巨噬细胞NO释放和二甲苯引起的小鼠耳廓肿胀,且存在一定的剂量依赖性。结论秦艽和龙胆挥发油在体内外均显示了出色的抗炎活性,有望从中分离新的抗炎活性成分。Objective To study the anti-inflammatory activities of essential oils from the roots of G.crassicaulis Duthie ex Burkand G.scabra Bunge in vivo and in vitro.Methods The essential oils were extracted by supercritical fluid extraction（SFE）,and the chemical components of the essential oils were analyzed by GC-MS.In vitro and in vivo anti-inflammatory activities of the essential oils were investigated with a cellular model of LPS-stimulated RAW264.7 cell and xylene-induced ear edema in mice.Results The essential oils were transparent and full aroma,and 29,40 compounds were identified from G.crassicaulis and G.scabra respectively.The essential oils of G.crassicaulis and G.scabra（GCEO and GSEO） showed a good performance of inhibiting NO production and xylene-induced ear edema in mice with a dose-dependent manner.Conclusion GCEO and GSEO had excellent anti-inflammatory activities in vivo and in vitro,and thus had great potential to be further studied on the active anti-inflammatory ingredients.

关 键 词：秦艽 龙胆 挥发油 抗炎作用 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]