水稻OsOle1基因的克隆及其遗传转化  

Cloning and Genetic Transformation of OsOle1 Gene in Rice

作　　者：姚清国[1] 李晓芹[1] 李晓兵[1] 段书德[1] 

机构地区：[1]石家庄学院化工学院,河北石家庄050035

出　　处：《安徽农业科学》2011年第21期12641-12642,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]克隆水稻OsOle1基因并对其进行遗传转化。[方法]通过RT-PCR方法对OsOle1基因进行克隆,将克隆出的OsOle1基因连接到pCAMBIA 1300上构建其超表达载体,并通过农杆菌介导对水稻愈伤进行了遗传转化。[结果]克隆出的OsOle1基因全长498bp,编码165个氨基酸,成功构建了其超表达载体Ub::OsOe1-GUS,最终得到了转基因株系。[结论]为OsOle1基因的功能研究奠定了基础。[Objective] The cloning and transformation of rice OsOle1 gene were conducted in the research.[Method] OsOle1 gene was cloned by RT-PCR.The amplified OsOle1 was then ligased to pCAMBIA 1300 to construct GUS overexpression vector.Then the Agrobacteriummediated method was used in rice callus transformation.[Result]The full-length of OsOle1 gene was 498 bp and it encoded 189 amino acids.The over-expression vector Ub∷OsOe1-GUS was prepared and transgenic plants were successfully obtained.[Conclusion]The transgenic lines laid the foundation for the function research of OsOle1.

关 键 词：水稻 基因克隆 遗传转化 

分 类 号：Q291[生物学—细胞生物学]