朱槿叶色素的提取与分离  

Extract and Separation of Hibiscus Leaf Pigment

作　　者：彭梦侠[1] 刘小玲[1] 杨丹敏[1] 罗婷媚[1] 

机构地区：[1]嘉应学院化学与环境学院,广东梅州514015

出　　处：《实验科学与技术》2011年第4期1-3,17,共4页Experiment Science and Technology

基　　金：第四批高等学校特色专业建设资助项目(教高函[009]16号);嘉应学院第七批新世纪教育教学改革工程重点项目(009-01-07)

摘　　要：开发了以朱槿叶为原料进行薄层色谱实验的新方案。选取以成熟的朱槿叶为原料,用石油醚∶乙醇为3∶2作为朱槿叶色素的提取剂,用薄层色谱对朱槿叶所含的色素进行了分离。研究了多种展开剂对色素分离的影响,选择出最佳的层析条件。以石油醚∶丙酮为7∶3作为展开剂,分出5个清晰的、集中的色斑,按Rf值的顺序由大到小分别是:黄色、灰色、蓝绿色、草绿色、亮黄色。对各种色素进行了紫外光谱表征,对其成分进行了初步的鉴定。The new experiment plan was developed with the hibiscus leaves as raw materials,with petroleum ether and alcohol（The volume ratio is 3∶2） as extractant,for thin layer chromatography experiments and separation.The experiment selected the mixture solution of petroleum ether and acetone（The volume ratio is 7∶3） as expanding agent,utilized the best conditions of these solutions.The separating result was five centralized and clear spots,descending order according to Rf values are yellow,gray,blue green,grass green,and bright yellow,respectively.Various pigments were characterized by UV spectrum,and its components were initially identified.

关 键 词：色素 朱槿叶 薄层色谱分析 

分 类 号：O657.7[理学—分析化学]