HPLC法同时测定发酵液中纽莫康定A_0与B_0的含量  

Simultaneous Determination of Content of Pneumocandin A_0 and B_0 in Fermentation Broth by HPLC

作　　者：张雪霞[1] 李宁[1] 李晓露[1] 林旸[1] 张金娟[1] 林毅[1] 蒋沁[1] 

机构地区：[1]微生物药物国家工程研究中心河北省工业微生物代谢工程技术研究中心华北制药集团新药研究开发有限责任公司,河北石家庄050015

出　　处：《化学与生物工程》2011年第8期88-90,共3页Chemistry & Bioengineering

基　　金：国家"重大新药创制"科技专项(2010ZX09401-403)

摘　　要：采用HPLC方法同时测定发酵液中纽莫康定A0与B0的含量。色谱柱为SepaxSapphire C18(4.6 mm×250mm,5μm),流动相为甲醇-水(体积比45∶55,内含0.1‰三氟乙酸),流速为1.0 mL.min-1,紫外检测波长为214nm。结果表明,纽莫康定A0与B0的浓度分别在47.5～191.4μg.mL-1和38.4～153.6μg.mL-1的范围内,与峰面积呈良好的线性关系(R分别为0.9997和0.9998);检测灵敏度分别为6.86 ng、5.95 ng;平均回收率分别为99.15%(RSD=0.96%,n=9)和99.31%(RSD=0.98%,n=9)。该方法简便、准确、重现性好,可用于发酵液中纽莫康定A0与B0含量的测定。Simultaneous determination of content of pneumocandin A0 and B0 in fermentation broth by HPLC was studied. The HPLC conditions consisted of a SepaxSapphire C18 column （4.6 ram）〈 250 mm,5 μm）,mobile phase of methanol-H20 （45 ： 55） containing 0.1%0 trifluoracetic acid with a flow rate of 1.0 mL ·min-1 and detection wavelength at 214 nm. The linearity of pneumocandin A0 and B0 concentration was shown in the range of 47.5-191.4 /,g ~ mL-1 （R：0. 9997） and 38.4-153.6 /,g · mL-1 （R=0. 9998） ;the sensitivity of pneumocandin Ao and B0 was 6.86 ng, 5.95 ng;the average recovery of pneumocandin Ao and Bo was 99.15~//00 with RSD =0.96%（n=9） and 99.31% with RSD=0. 980% （n=9）,respectively. The HPLC method, which was simple and accurate with good repeatability, could be applied to simultaneous determination of content of pneumocan- din Ao and Bo in fermentation broth.

关 键 词：高效液相色谱法 纽莫康定A0 纽莫康定B0 

分 类 号：O657.72[理学—分析化学]