苹果周期蛋白依赖性蛋白激酶亚基基因MdCKS表达载体的构建  

Construction of Plant Expression Vector of Apple MdCKS Gene

作　　者：焦其庆[1] 陈学森[1] 郑巧娜[1] 崔美[1] 陈晓流[1] 

机构地区：[1]山东农业大学园艺科学与工程学院/作物生物学国家重点实验室,山东泰安271018

出　　处：《山东农业科学》2011年第8期1-3,共3页Shandong Agricultural Sciences

基　　金：山东省自然科学基金项目(Y2007D26)资助

摘　　要：根据GenBank上与周期蛋白依赖性蛋白激酶基因相关序列,从苹果MdCKS基因编码区设计带有XbaⅠ和SalⅠ酶切位点的引物,以富士MdCKS基因的cDNA为模板将PCR扩增片段连接到pMD18-T Sim-ple Vector上,测序正确后,再连接到植物表达载体pBI121上,经过抗性筛选和测序验证,成功构建了MdCKS基因表达载体。并通过电击转化法导入农杆菌LBA4404,以备将来用于番茄转化。Based on the sequences of the cyclin - dependent kinase genes in GenBank, the primers were designed for PCR with the restriction enzyme sites of Xba I and Sal I from the ORF of the MdCKS gene for PCR, The template was the cDNA of MdCKS from Fuji apple. The PCR fragment was firstly inserted into the pMD18-T Simple Vector, and then inserted into the plant expression vector pBI121 after sequenced. The plant expression vector of MdCKS gene was constructed successfully by resistance selection and sequence confirmation. The recombinant plasmid was transformed into Agrobacterium tumfaciens LBA4404 by electroperation for genetic transformation of tomato.

关 键 词：苹果 MdCKS 表达载体 

分 类 号：Q786[生物学—分子生物学]