奶制品中二恶英类化合物两种提取方法的比较  

COMPARISON OF TWO EXTRACTION METHODS FOR DETERMINATION OF DIOXIN AND DIOXIN-LIKE PCBS IN MILK AND MILK PRODUCTS

作　　者：蒋友胜[1] 张建清[1] 周健[1] 黄海燕[1] 李胜浓[1] 

机构地区：[1]深圳市疾病预防控制中心,深圳518020

出　　处：《现代预防医学》2011年第17期3533-3535,共3页Modern Preventive Medicine

摘　　要：[目的]比较奶和奶制品中二恶英类化合物索氏抽提法和液液萃取法。[方法]采集市售4种纯牛奶和3种奶粉,分别通过索氏抽提和液液萃取,再用酸性硅胶处理,利用硅胶柱和铝柱净化,用高分辨气相色谱/高分辨质谱检测分析PCDDs/Fs和PCBs。[结果]索氏抽提方法对牛奶和奶制品中脂肪不能提取完全,得到的二恶英类化合物检测结果不能真实反映牛奶和奶制品中二恶英类化合物污染情况。液液萃取法能完全萃取牛奶和奶制品中脂肪以及溶于脂肪中的二恶英类化合物,得到的结果能真实反映牛奶和奶制品中二恶英类化合物污染情况。[结论]液液萃取法是适用于检测牛奶和牛奶制品中二恶英类化合物的检测方法。[Objective]To compare Soxhlet extraction with liquid-liquid extraction for determination of dioxin and dioxin-like PCBs in milk and milk products.[Methods]The total 4 kinds of milk and 3 kinds of milk powders were collected in the market,and were individually extracted by Soxhlet extraction and liquid-liquid extraction,and then treated by acidic silica gel,cleaned up through silica gel and alumina column and determined by HRGC/HRMS.[Results]Soxhlet extraction couldn＇t completely extract the full fat in milk and milk products,the result couldn＇t really reflect the pollution of dioxin and dioxin-like PCBs in milk and milk products.Liquid-liquid extraction could completely extract the full fat in milk and milk products,and the results were reliable.[Conclusion]Liquid-liquid extraction is a suitable method for determination of dioxin and dioxin-like PCBs in milk and milk products.

关 键 词：二恶英 多氯联苯 牛奶 HRGC/HRMS 

分 类 号：R115[医药卫生—公共卫生与预防医学]