活化溴酸钾-溴酚蓝-钒催化体系动力学光度法测定柠檬酸  

Determination of citric acid by activation catalytic reaction system of potassium bromate-bromophenol blue-Vanadium(V) dynamic spectrophotometric

作　　者：彭圣娟[1] 丁素芳[2] 

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]淮北师范大学化学与材料科学学院,安徽淮北235000

出　　处：《中国卫生检验杂志》2011年第8期1845-1846,共2页Chinese Journal of Health Laboratory Technology

基　　金：安徽省高等学校省级自然科学研究项目(KJ2010A305);安徽省科技厅自然科学基金项目(11040606M42)

摘　　要：目的:建立动力学光度法测定柠檬酸的新方法。方法:在酸性介质中,柠檬酸对溴酸钾、溴酚蓝、钒(V)催化体系具有强烈的活化作用,在最大吸收波长587 nm及最佳实验条件下,测定催化体系和活化催化体系吸光度的变化,建立测定柠檬酸的新方法。结果:测定柠檬酸的线性范围为0.12 mg/ml～97.00 mg/ml,方法的检出限为4.026×10-7 g/ml。结论:该方法直接用于柠檬复合果汁中柠檬酸的测定,结果满意。Objective：To establish a kinetic spectrophotometric method for the determination of citric acid.Methods：In acid medium,citric acid had a remarkable activation effect on the catalytic system of potassium bromate,bromophenol blue and vanadium（V）,based on which,kinetic spectrophotometric method for the determination of citric acid was established.The absorbance of the catalytic systems and the activation catalytic systems were measured at the maximum absorption of 587 nm and the optimum conditions.Results：The linear range of the determination was between 0.12 mg/ml～97.00 mg/ml and the detection limit was 4.026 ×10-7 g/ml.Conclusion：The method has been used directly for the determination of citric acid in drinks with the satisfactory results.

关 键 词：柠檬酸 溴酸钾 溴酚蓝 钒 催化体系 活化作用 

分 类 号：O657.32[理学—分析化学]