亚丁湾海域海水理化性质和主要细菌调查  

The investigation of physicochemical characteristics and major bacterial species of seawater in Gulf of Aden area

作　　者：韩善桥[1] 巴剑波[2] 赵晓航[1] 姜涛[1] 朱智明[1] 安怀杰[1] 陈双红[2] 徐雄利[2] 何立东[1] 

机构地区：[1]海军总医院,北京100048 [2]海军医学研究所,上海200433

出　　处：《中国卫生检验杂志》2011年第8期2036-2037,2040,共3页Chinese Journal of Health Laboratory Technology

基　　金：国家自然科学基金(91029725);总后医药卫生科研计划(06MB022);海后司令部科研计划(07-3309)资助

摘　　要：目的:了解亚丁湾海域海水主要理化特性、细菌种类及其对抗菌药物的敏感性。方法:采集亚丁湾海域不同地理位置海水样本;用自动生化分析仪测定海水主要理化指标;海水经增菌培养和细菌分离,对所分离细菌进行菌株鉴定及其对不同抗菌药物敏感性试验。结果:亚丁湾海域海水Na+、K+、Cl-和Ca2+离子含量分别为525.0±14.0、12.5±0.25、86.8±16.1和11.7±0.3 mmol/L;从8份不同地理位置海水水样中共分离和培养细菌菌株80株,均鉴定为斯氏假单胞菌;药敏实验分析表明该海域斯氏假单胞菌对头孢呋辛、头孢他啶、奈替米星和环丙沙星等18种临床常用抗菌药物均敏感。结论:亚丁湾海域海水理化性质和常见致病菌具有特殊性,对指导该海域从业人员健康保障和维护具有科学意义。Objective：To investigate the main physicochemical characteristics,bacterial species and their antimicrobial susceptibility of the seawater in Gulf of Aden area.Methods：Automatic biochemical analyzer was used to detect seawater physicochemical indexes.Total bacteria of 8 water samples collected from different regions of Gulf of Aden area were incubated,separated and species identified.The microbial sensitivity tests were performed for major species of bacteria via a Kirby-Bauer method.Results：The ion concentration of sodium,potassium,chlorine and calcium were found 525.0±14.0,12.5±0.2,586.8±16.1 and 11.7±0.3 mmol/L respectively in the seawater of Gulf of Aden.Total 80 strains were isolated from 8 samples of seawater and only one bacteria species,Pseudomonas stutzeri,were finally identified.The analysis of Pseudomonas stutzeri susceptibility showed that it was sensitive under treatment of 18 kinds of routine antimicrobial agents in clinic,incuding cefazolin,cefuroxime,ceftazidime,ceftriaxone,cefoperazone,cefepime,ampicillin,piperacillin,netilmicin,amikacin,ciprofloxacin,levofloxacin,aztreonam,trimethoprim/sulfamethoxazole,cefoperazone/sulbactam,piperacillin/tazobactam,imipenem and meropenem.Conclusion：The main physicochemical characteristics,common pathogenic bacteria and their antimicrobial susceptibility of the seawater had particular features in Gulf of Aden area,which may have scientific significances for health contrls of people who were working in this area.

关 键 词：海水 亚丁湾海域 细菌 抗菌药物 

分 类 号：R378[医药卫生—病原生物学]