中国荷斯坦种公牛亲子鉴定及个体识别信息库的建立  

Establishment of a Genotype Database for Genetic Testing Using Microsatellites in Chinese Hostein Bulls

作　　者：杨超[1] 张毅[1] 石万海[2] 陈绍祜[3] 张胜利[1] 张沅[1] 

机构地区：[1]中国农业大学动物科技学院,北京100193 [2]北京奶牛中心,北京100192 [3]中国奶业协会奶牛数据中心,北京100192

出　　处：《中国奶牛》2011年第16期1-5,共5页China Dairy Cattle

基　　金：国家科技支撑计划(2006BAD04A01)(2011BAD28B02);948项目(2010-C14);行业科研专项(nyhyzx07-036-13);国家奶牛产业技术体系;北京市科技计划(D08060500070801)

摘　　要：为了构建基于微卫星标记的中国荷斯坦种公牛亲子鉴定及个体识别技术平台,本研究选取国际动物遗传学会(ISAG)推荐的12个微卫星标记(BM1824,BM2113,SPS115,ETH10,TGLA122,ETH225,INRA023,TGLA126,TGLA227,BM1818,ETH003和TGLA53),采用荧光标记毛细管电泳方法,对571头荷斯坦种公牛进行基因型检测。统计结果显示,标记的平均多态信息含量为0.700,平均杂合度为0.735,属于高多态性标记;12个标记的累积排除概率大于0.996,累积一致性概率为4.9×10-13,将其运用于亲子鉴定和个体识别具有很强的检测效率。此外,通过分析每个标记的检测信号峰图特征,将检测结果与商业化试剂盒进行比较以及利用ISAG参考DNA样品进行等位基因统一命名等手段,建立了标准化的微卫星等位基因分型方法,并初步建立起中国荷斯坦种公牛遗传检测信息库。To develop a parentage analysis and identity testing system for Chinese Holstein bulls, twelve microsatellites（BM1824, BM2113, SPSll5, ETH10, TGLA122, ETH225, INRA023, TGLA126, TGLA227, BM1818, ETH3 and TGLA53）recommend by International Society of Animal Genetics （ISAG） were used to examine 571 Holstein bulls with capillary electrophoresis. Results showed that these markers were high polymorphic, whh mean polymovphic information content of 0.700 and expeeted heterozygosity of 0.735, respectively. The cumulated exclusion probability and probability of identity was 0.997 and 4.9 ×10^-13 respectively, indicating their high power for paternity analysis and identity testing. In addition, the peak pattern of each locus was characterized so as to standardize the allelic assignment. This study facilitated future application of paternity testing and the development of a genotype database of Chinese Hostein bulls.

关 键 词：遗传标记 种公牛 亲子鉴定 排除概率 一致性概率 

分 类 号：S823.2[农业科学—畜牧学]