刺五加苷B、苷E在体外大鼠肠道菌群中的代谢  

Metabolism of Eutheroside B and E in Rat Intestinal Flora in vitro

作　　者：刘树民[1] 杨补科[1] 卢芳[1] 白云[2] 牟洪[1] 马丁[1] 

机构地区：[1]黑龙江中医药大学中医药研究院,哈尔滨150040 [2]黑龙江中医药大学基础医学院

出　　处：《中国药师》2011年第8期1075-1077,1081,共4页China Pharmacist

基　　金：国家科技重大专项课题(编号:2009ZX09103-329);国家自然科学基金青年科学基金项目(编号:30901974);教育部"春晖计划"合作科研项目(编号:Y-30);教育部"春晖计划"合作项目(编号:Z2009-1-15032)

摘　　要：目的:观察体外大鼠肠道菌群对刺五加苷B、苷E的代谢。方法:收集大鼠新鲜粪便在厌氧培养基37℃培养24h,分别加入刺五加苷B、刺五加苷E,培养后加甲醇提取离心,取上清液采用HPLC及UPLC/MS方法对代谢成分进行分离和定性分析。结果:刺五加苷B、苷E在大鼠粪便孵育液中代谢,24h后样本中能检测出较高浓度代谢物。在离体条件下,刺五加苷B、苷E可以被大鼠的肠道菌群代谢,经过UPLC/MS的检测,刺五加苷B代谢物的分子离子峰[M+H]^+为193.08,推测为刺五加苷B的苷元再脱一分子水;刺五加苷E代谢物的分子离子峰[M+H]^+为417.17,推测为刺五加苷B的苷元。结论:刺五加苷B、苷E可以被大鼠肠道菌群代谢。Objective：To study and compare the metabolism of eleutheroside B and E in the rat intestinal flora in vitro.Method： After the rat fresh feces was collected and anaerobically cultured for 24 h at 37℃,eleutheroside B and E was added and cultured,respectively. After extracted by methnol,the main metabolites of eleutheroside B and E were separated and analyzed by HPLC and UPLC/ MS.Result：In vitro,eleutheroside B and E were easily metabolized by the intestinal flora and the main metabolites in incubation medium were in high concentration after 24 h.The metabolite of eleutheroside B with molecular ion peak[M＋H]^＋ of 193.08 was identified to be its aglycon with a molecular water dehydrated,and the metabolite of eleutheroside E with molecular ion peak[M＋H]^＋ of 417.17 was identified to be its aglycon.Conclusion：In vitro,eleutheroside B and E were metabolized by the flona.

关 键 词：UPLC/MS 刺五加苷B 刺五加苷E 肠道菌群 

分 类 号：R969.1[医药卫生—药理学]