加味生脉饮注射液对内毒素休克大鼠肺组织肿瘤坏死因子-α、白细胞介素-1β和核转录因子-κB含量的影响  

Influence of Supplemented Shengmaiyin Injection on Contents of TNF-α,IL-1β and NF-κB in Lung Tissue of Endotoxic Shock Rats

作　　者：王新芳[1] 张宇忠[2] 任丽薇[2] 贾旭[2] 李姝玉[2] 张银柱[2] 杨莹[2] 庞丹丹[2] 

机构地区：[1]首都医科大学燕京医学院生理学与病理生理学教研室,北京101300 [2]北京中医药大学基础医学院病理教研室

出　　处：《中国药师》2011年第8期1078-1081,共4页China Pharmacist

基　　金：国家自然科学基金资助项目(编号:30672737)

摘　　要：目的:观察加味生脉饮注射液对内毒素休克肺组织TNF-α、IL-1β和NF-κB表达的影响。方法:静脉注射脂多糖(LPS,8.00mg·kg^(-1))造成内毒素休克模型,用加味生脉饮注射液作干预治疗,酶联免疫吸附法检测上述因子在假手术组、模型组、加味生脉饮组、阳性对照组等各组大鼠肺组织内的表达。结果:模型组肺损伤较重,TNF-α、IL-1β和NF-κB含量明显升高(P<0.05);加味生脉饮组和阳性对照组肺损伤较轻,TNF-α、IL-1β和NF-κB含量明显降低(P<0.05)。结论:加味生脉饮注射液能减轻内毒素休克时肺损伤和TNF-α、IL-1β和NF-κB表达,提示该药对内毒素休克肺损伤的保护作用可能是通过调控NF-κB通路过度激活而实现。Objective：To investigate the effects of supplemented Shenmaiyin injection on the expression of TNF-α,IL-1βand NF-κB in lung tissue of endotoxic shock rats.Method：Rats were randomly divided into sham operation group,model group,supplemented Shenmaiyin group and positive control group.Endotoxic shock rat model was established by intravenous injection lipopolysaccharide （LPS,8.00 mg·kg^-2）.TNF-α,IL-1βand NF-κB of the four groups were detected by enzyme-linked immunosorbnent assay.Result： Lung injury of the model group was severe with increased expressions of TNF-α,IL-1βand NF-κB（P0.05）,while that of supplemented Shenmaiyin group and positive control group was slight with decreased expressions of TNF-α,IL-1βand NF-κB（P0.05）.Conclusion： Supplemented Shenmaiyin injection can alleviate the lung injury and reduce the expressions of TNF-α,IL-1βand NF-κB.The protection of supplemented Shenmaiyin injection to endotoxin shock lung injury may be reached by regulating and controlling excessive activation of NF-κB pathway.

关 键 词：内毒素休克 肺损伤 加味生脉饮注射液 核转录因子-ΚB 细胞因子 

分 类 号：R285.5[医药卫生—中药学]