HPLC法测定血浆中法罗培南浓度的不确定度分析  

Uncertainty Evaluation in the Determination of Faropenem in Plasma by HPLC

作　　者：杨娟[1] 沈金芳[1] 孙黎[1] 苏克剑[1] 

机构地区：[1]上海交通大学医学院附属仁济医院临床药理研究室,上海200001

出　　处：《中国药师》2011年第8期1106-1109,共4页China Pharmacist

基　　金：国家科技部十一五计划"重大新药创制"科技重大专项基金资助项目(编号:2008ZX09312)

摘　　要：目的:对HPLC法测定血浆中法罗培南浓度的测量不确定度进行分析,以找到影响不确定度的因素,为评价检测报告提供科学依据。方法:根据《测量不确定度评定与表示》,并参考《化学分析中不确定度的评估指南》,对血浆中法罗培南含量测定进行测量不确定度的分析与评定。结果:标准不确定度为1.28μg·ml^(-1),扩展不确定度为2.56μg·ml^(-1),含量测定结果可表示为(10.14±2.56)μg·ml^(-1)。结论:建立的不确定度评估方法可用于HPLC法测定血浆中法罗培南含量的不确定度评估。测量不确定度的评定方法的确立对于血浆中法罗培南含量测定方法标准的研究具有重要意义。Objective：To evaluate the uncertainty in the determination of faropenem in plasma by HPLC and find the influencing factors in order to provide a scientific base for the test report evaluation.Method：The uncertainty in the determination of faropenem in plasma was analyzed and evaluated according to the＂Evaluation and Expression of Uncertainty Measurement＂and the＂Manual of Uncertainty Evaluation in Chemical Analysis＂.Result：The standard uncertainty was 1.28μg·ml^-1,and the expanded uncertainty was 2.56μg·ml^-1.The result of the determination could be expressed as（10.14±2.56）μg·ml^-1.Conclusion：The established uncertainty assessment method can be used in the uncertainty evaluation in the determination of faropenem in plasma by HPLC and is valuable in the determination standard study of faropenem in plasma.

关 键 词：测量不确定度 高效液相色谱法 法罗培南 药物动力学 

分 类 号：R917[医药卫生—药物分析学]