Screening and Identifying of Nephrotoxic Compounds in Lithospermum erythrorhizon Using Live-cell Fluorescence Imaging and Liquid Chromatography Coupled with Tandem Mass Spectrometry  

作　　者：ZHAO Xiao-ping JIN Ye-cheng ZENG Xing ZHANG Bo-li ZHANG Yu-feng 

机构地区：[1]Tianjin State Key Laboratory of Modern Chinese Medicine,Tianjin University of Traditional Chinese Medicine, Tianjin 300193, P. R. China [2]College of Preclinical Medicine, Zhejiang Chinese Medical University Hangzhou 310053, P. R. China,＂ [3]College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, P. R. China

出　　处：《Chemical Research in Chinese Universities》2011年第4期562-565,共4页高等学校化学研究（英文版）

基　　金：Supported by the National Key Scientific and Technological Project of China(No.2009ZX09502-012);the Research Fund for the Doctoral Program of Higher Education of China(No.20090101110126);the Zhejiang Province Science and Technology Plan Project,China(No.2008C23065)

摘　　要：In order to identify the potential nephrotoxic compounds in traditional Chinese medicine Lithospermum erythrorhizon,it was separated into serial fractions according to their polarities.An in vitro method was utilized to determine the nephrotoxicity of these fractions with the help of fluorescence image analysis.As a result,the primary fraction A05 and its secondary fractions C06 ＂C09 and C12 ＂C14 were found to have significant toxicity to LLC-PK1 cell line,as determined by the survive rate less than 20% after they were treated with these fractions.These potential nephrotoxic fractions were further analyzed by multistage and high resolution mass spectrometry.The main compounds in these fractions were tentatively identified to be acetylshikonin,isobutyrylshikonin,β,β＇-dimethyla-cryloylshikonin,and isovalerylshikonin,which may bring nephrotoxicity.In order to identify the potential nephrotoxic compounds in traditional Chinese medicine Lithospermum erythrorhizon,it was separated into serial fractions according to their polarities.An in vitro method was utilized to determine the nephrotoxicity of these fractions with the help of fluorescence image analysis.As a result,the primary fraction A05 and its secondary fractions C06 ＂C09 and C12 ＂C14 were found to have significant toxicity to LLC-PK1 cell line,as determined by the survive rate less than 20% after they were treated with these fractions.These potential nephrotoxic fractions were further analyzed by multistage and high resolution mass spectrometry.The main compounds in these fractions were tentatively identified to be acetylshikonin,isobutyrylshikonin,β,β＇-dimethyla-cryloylshikonin,and isovalerylshikonin,which may bring nephrotoxicity.

关 键 词：Traditional Chinese medicine Nephrotoxic compound Liquid chromatography/ion-trap mass spectrome-try（LC/IT-MS） Liquid chromatography/time-of-flight mass spectrometry（LC/TOF-MS） Lithospermum erythrorhizon 

分 类 号：O657.63[理学—分析化学] TN206[理学—化学]