Anti-tumor Effects of a Recombinant Fowlpox Virus Expressing Apoptin on Human Cervical Carcinoma in Vivo and in Vitro  

作　　者：ZHU Ji-hong LI Xiao SUN Li-li ZHANG Mu-chun KAN Shi-fu LIU Lei HUANG Hai-yan YANG Guo-hua PIAO Bing-guo JIN Ning-yi 

机构地区：[1]Department of Gynecology and Obstetrics, the First Hospital of Jilin University, Changchun 130021, P. R. China [2]Laboratory of Genetic Engineering of PLA, Academy of Military Medical Sciences, Changchun 130062, P. R. China [3]Department of Head and Neck Surgery, Tumor Hospital of Jilin Province, Changchun 130012, P R. China [4]Department of Urology, China-Japan Union Hospital of Jilin University, Changchun 130033, P. R. China [5]Department of Gastroenterology, the First Hospital of Jilin University, Changchun 130021, P. R. China

出　　处：《Chemical Research in Chinese Universities》2011年第4期646-650,共5页高等学校化学研究（英文版）

基　　金：Supported by the National High-Tech Research and Development Program of China(No.2007AA021004);the National Basic Research Program of China(No.2005CB523005);the National Science and Technology Major Project of China (Nos.2008ZX10004-015,2009ZX08006-002B);the National Natural Science Foundation of China(No.30771609)

摘　　要：Apoptin is a chicken anemia virus-derived,p53-independent,bcl-2-insensitive apoptotic protein with the ability to specifically induce apoptosis of various human tumor cells,but not of normal diploid cells.To explore the application of apoptin in tumor gene therapy,we used a recombinant fowlpox virus expressing apoptin protein （vFV-Apoptin） to investigate the anti-tumor effectes of vFV-Apoptin on human cervical carcinoma（HeLa） cells in vivo and in vitro through 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide（MTT） assay,acridine orage/ethidium bromide（AO/EB） and annexin V staining test,respectively.The results show that vFV-Apoptin inhibites the proliferation of HeLa cells in vitro through inducing the apoptosis of HeLa cells,and the inhibition effect of vFV-Apoptin has a dose-effect and time-effect relationship.The results of animal models show that vFV-Apoptin significantly inhibits tumor growth,extends the lifespan of animals and improves the mean survival.Experimental results indicate that vFV-Apoptin has a potential application in the tumor gene therapy.Apoptin is a chicken anemia virus-derived,p53-independent,bcl-2-insensitive apoptotic protein with the ability to specifically induce apoptosis of various human tumor cells,but not of normal diploid cells.To explore the application of apoptin in tumor gene therapy,we used a recombinant fowlpox virus expressing apoptin protein （vFV-Apoptin） to investigate the anti-tumor effectes of vFV-Apoptin on human cervical carcinoma（HeLa） cells in vivo and in vitro through 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide（MTT） assay,acridine orage/ethidium bromide（AO/EB） and annexin V staining test,respectively.The results show that vFV-Apoptin inhibites the proliferation of HeLa cells in vitro through inducing the apoptosis of HeLa cells,and the inhibition effect of vFV-Apoptin has a dose-effect and time-effect relationship.The results of animal models show that vFV-Apoptin significantly inhibits tumor growth,extends the lifespan of animals and improves the mean survival.Experimental results indicate that vFV-Apoptin has a potential application in the tumor gene therapy.

关 键 词：Apoptin gene Chicken anemia virus Human cervical carcinoma Anti-tumor effect 

分 类 号：Q939.139[生物学—微生物学] S852.65[农业科学—基础兽医学]