银(Ⅰ)邻菲罗啉体系共振瑞利散射测定肝素钠  

Determination of Heparin with Ag(Ⅰ)-Phenanthroline System by Resonance Rayleigh Scattering Method

作　　者：黄伟涛[1] 梁娣[1] 罗红群[1] 李念兵[1] 

机构地区：[1]西南大学化学化工学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2011年第7期50-53,共4页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(20975083)

摘　　要：基于在pH=5.4的醋酸-醋酸钠缓冲溶液中,银(Ⅰ)可与邻菲罗啉形成配阳离子[Ag(Phen)2]+,进而与肝素钠结合形成离子缔合物,使溶液的RRS强度显著增强这一现象,建立了一种简便、快速、灵敏度高的检测肝素钠的方法,考察了方法的适宜反应条件、影响因素和共存物质的影响,对肝素钠的检出限为20 ng/mL,并将该方法应用于实际样品的测定.A novel method for the determination of heparin（Hep） with Ag（Ⅰ）-phenanthroline by resonance Rayleigh scattering has been proposed.In the medium of HAc-NaAc buffer solution（pH=5.4）,the interaction of heparin with [Ag（Phen）2]＋ can be occurred which greatly enhanced the RRS intensity at 398 nm.The scattering intensity is directly proportional to the concentration of heparin in the range of 0.04-6.0 μg/mL and its detection limit is 20 ng/mL.The optimum conditions and coexistent materials were discussed.The novel scattering method is satisfying when applied in the determination of heparin in actual sample.

关 键 词：共振瑞利散射 肝素钠 邻菲罗啉 银离子 

分 类 号：O657.3[理学—分析化学]