血清抗风疹IgG抗体检测方法的比较研究  

Comparative study of detection methods for serum anti-rubella IgG antibody

作　　者：储艳[1] 柴文清[1] 

机构地区：[1]上海生物制品研究所第六研究室,200052

出　　处：《国际生物制品学杂志》2011年第4期188-190,共3页International Journal of Biologicals

摘　　要：目的 比较微量血凝抑制（micro-hemagglutination inhibition,HI）试验与酶联免疫吸附测定（enzyme-linked immunosorbent assay,ELISA）检测血清抗风疹IgG抗体的敏感性和特异性,确定ELISA法检测血清抗风疹IgG抗体的可行性.方法 采集江苏地区506名8～15月龄健康儿童免疫前和麻疹-腮腺炎-风疹联合疫苗免疫后1个月的血清,共1012份血清,分别采用HI法和ELISA法检测血清抗风疹IgG.抗体,并采用卡方检验对两种方法的检测结果进行比较.结果 HI法和ELISA法的血清抗风疹抗体阳性检出率分别为50.30%和47.04%,两种方法的阳性检出率之间的差异无统计学意义（X2=1.780,P=O.182）.两种方法检出同为阴性的血清495份,同为阳性的血清476份,两种方法的检测符合率为95.95%.相关与回归分析显示,两种方法具有高度的正相关性（r=O.806,P〈0.001）.结论 可用ELISA法替代HI法进行血清抗风疹IgG抗体检测.Objective To compare the sensitivity and specificity for detecting serum anti-rubella IgG antibody between micro-hemagglutination inhibition （ HI） test and enzyme-linked immunosorbent assay （ ELISA） , and define the feasibility of ELISA for detecting serum anti-rubella IgG antibody. Methods Serum samples from 506 healthy children aged 8-15 months in Jiangsu Province were collected before vaccination and one month after vaccination with measles-mumps-rubella combined vaccine, and total 1012 serum samples were obtained. Serum anti-rubella IgG antibodies were detected by HI test and ELISA, respectively, and the detection results between two methods were compared by chi-square test. Results The positive detection rates of HI and ELISA antibodies were 50.30% and 47.04% , respectively, and no statistically significant difference in positive detection rate was found between the two methods （X2 = 1. 780, P =0. 182）. Four hundred and ninety-five serum samples were negative and 476 serum samples were positive by both assays. The coincidence rate of detection between two methods was 95.95%. Correlation and regression analysis showed there was high positive correlation between two methods（r=0. 806,P〈0.001）. Conclusion ELISA can replace HI test to detecting serum anti-rubella IgG antibody.

关 键 词：风疹病毒 抗体 微量血凝抑制试验 酶联免疫吸附测定 

分 类 号：R7[医药卫生—临床医学] R446