分子氨对黄河鲤鱼血清抗氧化反应的影响  

Effect of Ammonia on Antioxidant Responses in Serum of Yellow River Carps

作　　者：张红梅[1] 姜会民[1] 

机构地区：[1]菏泽学院山东省动物生理生化重点实验室,山东菏泽274015

出　　处：《西南大学学报（自然科学版）》2011年第8期88-93,共6页Journal of Southwest University(Natural Science Edition)

基　　金：山东省自然科学基金资助项目(ZR2010CM046)

摘　　要：为了探讨养殖水域中分子氨对水生生物的毒性效应,在前期毒性试验的基础上设置3个试验组(分子氨质量浓度分别为0.071,0.143,0.284mg/L)、1个对照组对黄河鲤鱼(Cyprinus carpio haematopterus Temmink etSchlegel)进行毒性试验,在试验的7,14,28,35d取样检测,测定试验鱼血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活力和丙二醛(MDA)质量浓度.结果表明:随着分子氨质量浓度的增加,黄河鲤鱼血清中SOD酶活力呈先升后降的趋势,质量浓度为0.28mg/L时,SOD活力低于对照组,GSH-Px的活力和MDA质量浓度呈现上升的趋势,方差分析表明分子氨极显著地提高血清MDA质量浓度(p<0.01),二者存在剂量—效应关系;随着分子氨暴露时间的延长,SOD活力呈下降趋势,MDA质量浓度呈上升趋势,方差分析差异不显著(p>0.05).在试验质量浓度范围内分子氨可降低鲤鱼抗氧化能力,且MDA可作为一种有效的生物标志物评价分子氨的毒性.To study the toxic effects of ammonia on the aquatic species,juveniles of Yellow River carp were assigned to 4 groups with an ammonia nitrogen concentration of 0（control）,0.071,0.143 and 0.284 mg/L respectively.Samples were taken on 7,14,28 and 35 days after the start of the treatments and SOD and GSH-Px activities and MDA content of the serum were determined.The results demonstrated that with increasing ammonia concentration,the activity of SOD in the serum first increased and then decreased,while the activity of GSH-Px and content of MDA showed a rising trend.Variance analysis indicated that ammonia exposure had a highly significant influence on the content of MDA（p〈0.01）.With the extension of ammonia exposure time,the activity of SOD declined and the content of MDA exhibited a rising trend.Variance analysis showed no significant difference（p〈0.05）.Within the concentration range of the test,ammonia reduced the oxidation resistance of carp,and MDA content could be used as an effective biomarker for toxicity assessment of ammonia in Yellow River carps.

关 键 词：分子氨 黄河鲤鱼 血清 超氧化物歧化酶 谷胱甘肽过氧化物酶 丙二醛 

分 类 号：S941.69[农业科学—水产养殖]