慢性乙型肝炎患者外周血CD8^+T细胞TCR Vβ基因亚家族克隆化的研究  

Study of clonality of TCR Vβ gene subfamilies on peripheral blood CD8^+ T lymphocyte in patients with chronic hepatitis B

作　　者：熊英[1] 宋玉国[1] 毕胜利[2] 谭岩[3] 

机构地区：[1]北华大学生命科学研究中心,吉林132011 [2]北华大学医学部,吉林132011 [3]吉林省人民医院医学诊治实验中心,长春130021

出　　处：《中国免疫学杂志》2011年第8期751-756,共6页Chinese Journal of Immunology

基　　金：吉林省卫生厅研究项目(20082082)

摘　　要：目的:分析慢性乙型肝炎(CHB)患者CD8+T细胞TCR Vβ基因亚家族克隆化特征。方法:采用逆转录-聚合酶链反应(RT-PCR)扩增8例CHB患者外周血CD8+T细胞TCR Vβ基因22个亚家族的CDR3区,基因扫描技术对TCR Vβ亚家族的克隆化进行鉴定。结果:基因扫描显示所有8例CHB患者CD8+T细胞TCR Vβ基因亚家族均出现一个或一个以上单克隆或寡克隆增生。Vβ8、Vβ11、Vβ12出现单克隆增生的频率相对较高。8例健康者TCR Vβ基因亚家族均为多克隆。结论:CHB患者外周血CD8+T细胞TCR Vβ亚家族存在克隆性增生。Objective：To analyze clonality of TCR Vβ gene subfamilies on peripheral blood CD8＋ T lymphocyte in patients with chronic hepatitis B.Methods：Expression of the 22 TCR Vβ gene subfamilies was analyzed using reverse transcription-polymerase chain reaction（RT-PCR）,which amplified the complementarity determining region 3（CDR3） of TCR Vβ gene on peripheral blood CD8＋T lymphocytes from 8 cases with chronic hepatitis B.PCR products were further analyzed by genescan to identify TCR Vβ subfamilies clonality.Results：Some TCR Vβ gene subfamilies showed monoclonal or oligoclonal expansion in the 8 CHB patients.Vβ8,Vβ11,Vβ12 had higher frequency of monoclonal expansion.All TCR Vβ gene families of CD8＋ T lymphocyte showed a Gaussian distribution in normal control cases.Conclusion：TCR Vβ subfamilies on CD8＋T lymphocyte of CHB patients during acute exacerbation showed clonal expansion.

关 键 词：慢性乙型肝炎 T细胞抗原受体 VΒ基因 互补决定区3(CDR3) 克隆化 

分 类 号：R593.62[医药卫生—内科学]