家蚕肠道产蛋白酶细菌的分离筛选与分子鉴定  

Isolation and molecular identification of intestinal protease producing bacteria from silkworm

作　　者：陈睿[1] 王在贵[1] 杨文静[1] 刘朝良[1] 张宏福[2] 唐湘方[2] 张琛[1] 陈敏[1] 赵二双[1] 李阿敏[1] 

机构地区：[1]安徽农业大学生命科学学院,安徽合肥230036 [2]中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京100193

出　　处：《中国微生态学杂志》2011年第8期681-683,688,共4页Chinese Journal of Microecology

基　　金：动物营养学国家重点实验室开放基金项目(2004DA125184F1104);安徽省自然科学基金项目(11040606M98);安徽农业大学大学生创新基金项目(2010235)

摘　　要：目的肠道细菌对家蚕具有重要的生理作用,探明家蚕肠道产蛋白酶细菌菌群对家蚕肠道细菌的高效开发与利用具有十分重要的意义。方法从菁松×皓月品种4龄家蚕肠液中分离与纯化肠道细菌,获得一株产高活力蛋白酶细菌,对该菌菌落进行形态学鉴定及菌体的显微镜检并结合16S rDNA方法进行了鉴定。结果该菌为革兰阴性杆菌,属于嗜麦芽寡养单胞菌(Stenotrophom onas maltophilia)。结论该产蛋白酶细菌产酶能力较高,可进一步开发研究并用作微生态制剂。Objective Intestinal bacteria play a significant physiological role on silkworm.Probing intestinal protease producing bacteria colony of silkworm is of great importance to efficient development and utilization of silkworm intestinal bacteria.Method Intestinal bacteria from Jingsong×Haoyue（aged 4） were isolated and purified to obtain high protease producing bacteria.The identified bacteria colony were examined by morphology and microscopy,combined with 16S rDNA method.Result This bacteria was Gram-negative and belonged to Stenotrophomonas maltophilia.Conclusion The identified bacteria is highly capable of producing protease,which can be further developed and investigated as a probiotic for silkworm.

关 键 词：家蚕 肠道菌 蛋白酶 16S RDNA 

分 类 号：Q814.1[生物学—生物工程]