当归挥发油不同部位对豚鼠离体气管平滑肌的作用及机制研究  

Study on the effects and mechanism of different components of Angelica naphtha on isolated tracheal smooth muscle in the guinea pig

作　　者：吴国泰[1] 任远[1] 王锋[1] 马骏[1] 王志旺[1] 

机构地区：[1]甘肃中医学院,甘肃兰州730000

出　　处：《甘肃中医学院学报》2011年第4期1-4,共4页Journal of Gansu College of Traditional Chinese Medicine

摘　　要：目的观察当归挥发油不同部位对豚鼠离体气管平滑肌的作用,并探讨其作用机制。方法用螺旋形环切法制作豚鼠离体气管平滑肌标本,通过二道生理记录仪描记张力变化曲线,观察当归挥发油不同部位对静息气管平滑肌及磷酸组胺(His)、氯化乙酰胆碱(Ach)预收缩气管平滑肌张力的影响。结果当归总挥发油(AN)与当归挥发油中性非酚性部位(A3)对豚鼠离体静息气管平滑肌均有明显的舒张作用(P<0.01);AN、当归挥发油酸性部位(A1)、当归挥发油酚性部位(A2)及A3均能显著减弱His所致豚鼠离体气管平滑肌的收缩作用,AN、A3能显著减弱Ach所致豚鼠离体气管平滑肌的收缩作用(P<0.01);A3能拮抗His,Ach引起的气管平滑肌痉挛,使其量效曲线幅度呈剂量依赖性降低。结论 A3能舒张豚鼠离体静息气管平滑肌,对抗His,Ach所致豚鼠离体气管平滑肌的收缩作用,初步推断A3属于组胺受体及M胆碱受体非竞争性拮抗剂。Objective To observe the effects of different components of Angelica naphtha on isolated tracheal smooth muscle in the guinea pig and discuss its related mechanism. Methods The isolated tracheal smooth muscle specimen of guinea pig were made by spiral annular shape resection. Tension variation curve was recorded by the two channels physiological recorder. The effects of different components of Angelica naphtha on resting tracheal smooth muscle and the tension of tracheal smooth muscle of pre contraction induced by histamine（His） and aeetylcholine（Ach） were observed. Results Angelica naphtha （AN） and neutral non phenol parts of Angelica naphtha （A3 ） had obvious diastolic effect on isolated tracheal smooth muscle in the guinea pig （P 〈 0.01 ）. AN ,acid parts of Angelica naphtha（ A1 ）, phenolic parts of Angelica naphtha（ A2 ） and A3could obviously abate the contraction effect of isolated tracheal smooth muscle in the guinea pig induced by His, AN and A3could obviously abate the contraction effect induced by Ach （P 〈 0.01 ）. A3 could resist the spasm of tracheal smooth muscle induced by His and Ach, and reduce the close-effect curve magnitude in a dose dependent manner. Conclusion A3 has diastolic effect on isolated tracheal smooth muscle in the guinea pig, can resist the contraction effect of isolated tracheal smooth muscle in the guinea pig induced by His and Ach. A3 could be the noncompetitive antagonists of histamine receptor and M muscarinic receptor.

关 键 词：当归挥发油 活性部位 气管平滑肌 舒张作用 

分 类 号：R285.5[医药卫生—中药学]