低温处理对耐寒粗枝木麻黄无性系生理指标的影响  

Effect of Low Temperature on Physiological Index of Cold-tolerant Casuarina glauca Clones

作　　者：何贵平[1] 卓仁英[1] 陈雨春 黄一青 彭柳青 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400 [2]浙江省上虞市世纪阳光园林绿化工程有限公司,浙江上虞312300 [3]浙江省慈溪市农业科学研究所,浙江慈溪315300

出　　处：《林业科学研究》2011年第4期523-526,共4页Forest Research

基　　金：国家"十一五"科技支撑项目"优质抗逆生态树种木麻黄新品种选育"专题(2006BAD01A1605);浙江省自然科学基金项目"利用冷特异诱导表达启动子培育超耐寒木麻黄新品种"(Y306072)

摘　　要：从经过低温(-5-6℃)冻害的粗枝木麻黄林分中选出较耐寒的单株,经无性繁殖成无性系,然后再选择出耐寒无性系。采用-2℃至-11℃低温处理后,2个较耐寒粗枝木麻黄无性系和对照的相对电导率、可溶性蛋白含量、MDA含量和SOD活性生理指标均表现为随着温度降低而升高的趋势,2个较耐寒无性系的相对电导率比对照低,而可溶性蛋白含量、MDA含量和SOD活性则比对照高,它们可用作鉴定粗枝木麻黄无性系耐寒性强弱的生理指标。慈36号无性系比慈24号无性系更耐寒。Cold-tolerant clones of Casuarina glauca were established through vegetative reproduction of the plants survived under low temperature（-5—6 ℃） stress.The physiological characteristics of two cold-tolerant clones and the control at 4 low-temperature gradient treatments from-2 ℃ to-11 ℃ were tested.The results showed that the relative conductivity,soluble protein content,MDA content and SOD activity of all clones increased with the temperature decrease.The relative conductivity of two cold-tolerant C.glauca clones were lower than that of the control,while the soluble protein content,MDA content and SOD activity were higher than that of the control.The relative conductivity,the soluble protein content,MDA content and SOD activity can be used as screening index for the cold tolerance identification of C.glauca.The Ci 36 clone was stronger cold-tolerant than Ci 24 clone.

关 键 词：粗枝木麻黄 耐寒 无性系 生理指标 

分 类 号：S722.36[农业科学—林木遗传育种]