碳酸盐碱度对钝顶螺旋藻生长的影响  

Effects of the different carbonate alkalinity concentrations on growth of Spirulina platensis

作　　者：陆建学[1] 么宗利[1] 夏连军[1] 应成琦[1] 周凯[1] 高露姣[1] 来琦芳[1] 

机构地区：[1]中国水产科学研究院东海水产研究所农业部海洋与河口渔业重点开放实验室,上海200090

出　　处：《生物学杂志》2011年第4期43-46,共4页Journal of Biology

基　　金：中央级公益性科研院所基本科研业务费专项资金(中国水产科学研究院东海水产研究所2008M03);中央级公益性科研院所基本科研业务费专项重点项目(中国水产科学研究院东海水产研究所2007Z03)

摘　　要：研究了不同碳酸盐碱度浓度条件下,钝顶螺旋藻OD560值、藻液pH值及碳酸盐碱度的变化。结果显示：培养4d后,4mg/L和8mg/L组生长缓慢且呈现下降趋势,OD560值均低于对照组,具有极显著差异（P〈0.01）,16mg/L和32mg/L组与对照组相比,无显著差异,而64mg/L组具有显著差异（P〈0.05）。第6~12d,4mg/L、8mg/L组和16mg/LOD560值均低于对照组,具有极显著差异（P〈0.01）,32mg/L组无显著差异,而64mg/L组在6~8d,具有显著差异（P〈0.05）,且在第10~12d,与其它实验组和对照组相比,表现出极显著差异性（P〈0.01）。NaHCO3与Na2CO3组成的缓冲体系,其pH值调节功能优于单一使用NaHCO3的对照组。在充气状态下,实验期间各组的碱度始终保持动态平衡。Spirulina platensis was cultivated,in comparative studies,using different concentrations of carbonate alkalinity.The influence of carbon concentrations of NaHCO3＋Na2CO3 on the growth of Spirulina platensis was studied in this paper.The results showed that there were absolutely significant differences in the OD560 values between treatment groups of 4mg/L,8mg/L,16mg/L and the control（P0.01）.The OD560 values in these groups were lower than that of the control from the sixth day to the twelfth day.There was no significant difference between treatment group of 32mg/L and the control,while significant difference was observed between 64mg/L and the control at the same time（P0.05）.Moreover,there were absolutely significant differences in the OD560 values between the treatment group of 64mg/L and other treatment groups and the control（P0.01）.The OD560 values in 64mg/L group were markedly higher than those of other treatment groups and the control from the tenth day to the twelfth day.It was pointed out that when compounds of NaHCO3 and Na2CO3 were added into the treatment group′s medium,the buffer functions for pH value were preferable to that of the control medium（modified CFTRI medium） in which single NaHCO3 was added.The results also indicated that the carbonate alkalinity of HCO-3＋ CO2-3 was maintained balance when CO2 was pumped into the medium.

关 键 词：钝顶螺旋藻 碳酸盐碱度 OD560值 PH值 

分 类 号：Q949.22[生物学—植物学]