不同培养基对水稻胚性愈伤组织诱导及分化的影响  

Rice callus induction and regeneration rate in different media

作　　者：刘书梅[1,2] 王姗姗[2] 辛晓云[2] 王玲玲[2] 朱正歌[2] 

机构地区：[1]石家庄科技工程职业学院,石家庄050800 [2]河北师范大学生命科学院,石家庄050016

出　　处：《生物学杂志》2011年第4期54-56,共3页Journal of Biology

基　　金：河北省教育厅基金项目(2007469);河北师范大学博士基金项目(L2001B01)资助

摘　　要：以水稻成熟胚为材料诱导愈伤组织,统计在不同基本培养基上的愈伤诱导率以及绿苗分化率,分析不同基本培养基及外源激素的含量和比例对愈伤组织生长及分化的影响。结果表明,试验材料对基本培养基具有选择性,MS培养基对籼稻种胚愈伤的诱导培养效果较好,NB培养基则更适合粳稻种胚愈伤的诱导培养;诱导继代培养基中加入多种氨基酸组合可有效提高出愈率和分化率,特别是粳稻的愈伤组织的诱导和分化需要多种氨基酸的共同作用;不同基因型水稻材料对激素和氨基酸组合的需求不同。Rice calli of two subspecies were induced from mature seeds,callus induction and regeneration rate were analyzed in this experiment.The result showed that NB medium was most suitable for Nipponbare（Oryza sativa L.subsp.Japonica）;MS,NB and LS medium were all appropriate for Longtepu（Oryza sativa L.subsp.Indica）.Some kinds of amino acids and hormone were necessary for Nippobare calli.It indicated that the rice calli induction and regeneration rate was related to rice variety.

关 键 词：水稻 成熟胚 愈伤组织 愈伤诱导率 绿苗分化率 

分 类 号：Q943.1[生物学—植物学]