雷公藤多苷对糖尿病肾病大鼠足细胞的保护作用  

Protective effect of TWP on the podocytes of diabetes nephropathy rats

作　　者：郑云[1] 郝丽[2] 潘梦舒[2] 丁楠[2] 

机构地区：[1]安徽医科大学第一附属医院急诊内科,合肥230022 [2]安徽医科大学第二附属医院肾脏内科,合肥230601

出　　处：《安徽医科大学学报》2011年第9期857-861,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:070413066)

摘　　要：目的探讨雷公藤多苷(TWP)对糖尿病肾病(DN)大鼠足细胞的保护作用。方法 SD大鼠60只,随机分为正常组(A组)、DN组(B组)与TWP治疗组(C组)。B、C组分别给予链脲佐菌素(STZ)一次性腹腔注射建立糖尿病大鼠模型。12周后观察24 h尿蛋白排泄量(UAER);HE染色观察肾组织病理变化;透射电镜检查足细胞超微结构变化;免疫荧光方法检测肾皮质Nephrin和Podocin蛋白表达。结果与A组比较,B、C组UAER明显增高(P<0.01),肾脏皮质Nephrin和Podocin蛋白表达减少,肾小球、小管间质、足细胞病变明显。与B组比较,C组UAER明显减少(P<0.01);肾脏皮质Nephrin和Podocin蛋白表达增高,肾小球、小管间质、足细胞病变明显减轻。结论 TWP对糖尿病大鼠足细胞病变具有保护作用,其部分机制与上调Nephrin和Podocin蛋白表达及改善足细胞病变有关。Objective To explore the protective effect of triptergium wilfordii polyglucoside（TWP） on the podocytes of diabetes nephropathy（DN） rats.Methods Sixty SD rats were randomly divided into normal control group（group A）,DN group（group B）,TWP group（group C）.Rats in group B and C were given streptozotocin（STZ） by intraperitoneal injection to establish animal model of diabetes.After 12 weeks,we observed UAER,kidney organization of pathological changes by HE staining,and ultrastructure changes of podocytes by TEM,and expression of the Nephrin and Podocin protein by immunofluorescence staining.Results Compared with group A,UAER were obviously higher（P0.01）;Nephrin and Podocin protein expressions in nephridial tissue were lower;Pathomorphological change in glomerulus,tubules and podocytes were observed in group B and C.Compared with group B,UAER were obviously lower（P0.01）;Nephrin and Podocin protein expressions in nephridial tissue were higher;Patho-morphologica change in glomerulus,tubules and podocytes showed a better improvement in group C.Conclusion The protective effect of TWP on the podocyte of DN rats may be partly referred with up-regulation of Nephrin and Podocin expressions and improved glomerular podocyte lesion.

关 键 词：糖尿病肾病 

分 类 号：R587.24[医药卫生—内分泌] R284[医药卫生—内科学]