玉屏风多糖对肝星状细胞中PDGF-BB、PDGFR-β的表达及ERK1/2通路的影响  

Effects of YPF-P on the expressions of PDGF-BB,PDGFR-β and ERK1/2 pathway in hepatic stellate cells

作　　者：曹荣娟[1] 李俊[1] 詹迪迪[1] 李祥胜[2] 

机构地区：[1]安徽医科大学药学院,安徽天然药物活性研究省级重点实验室,合肥230032 [2]安徽省食品药品检验所化学室,合肥230051

出　　处：《安徽医科大学学报》2011年第9期861-864,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:30973917);安徽省自然科学基金(编号:050431001)

摘　　要：目的通过观察玉屏风多糖(YPF-P)对肝星状细胞(HSC-T6)中血小板衍生生长因子BB(PDGF-BB)、血小板衍生生长因子BB受体(PDGFR-β)、c-fos基因表达的影响,从分子水平探讨其抗纤维化的作用机制。方法采用四甲基偶氮唑蓝(MTT)法观察不同浓度YPF-P(12.5、25、50、100、200 mg/L)分别在24、48、72 h对HSC增殖的影响,确定YPF-P最佳作用时间及浓度。RT-PCR法分析PDGF-BB、PDGFR-β、c-fos mRNA表达的变化。Western blot法检测ERK1/2和p-ERK1/2蛋白在不同干预情况下的表达。结果 YPF-P可明显抑制HSC-T6的细胞增殖以及下调HSC-T6中PDGF-BB、PDGFR-β、c-fos mRNA表达,并对HSC-T6中p-ERK1/2蛋白表达也有明显抑制作用。结论 YPF-P对PDGF诱导的大鼠HSC-T6增殖具有明显的抑制作用,其作用机制可能与抑制p-ERK1/2蛋白表达有关。Objective To investigate the molecular mechanism of anti-fibrosis of Yupingfeng pachy-polysaccharide（YPF-P） by observing the gene expressions of PDGF-BB,PDGFR-β and c-fos of rat hepatic stellate cell（HSC-T6）.Methods The influences of different concentrations of YPF-P（12.5,25,50,100,200 mg/L） on HSC proliferation were detected by MTT assay at 24,48 and 72 h,which calculated the inhibitory rates to determine the YPF-P＇s best role time and role concentration.The mRNA expressions of PDGF-BB,PDGFR-β and c-fos were observed by RT-PCR.The protein expressions of ERK1/2 and p-ERK1/2 under different interventions were detected by Western blot.Results After the intervention of different concentrations of YPF-P,the mRNA expressions of PDGF-BB,PDGFR-β,c-fos and the protein of p-ERK1/2 level were decreased obviously.Conclusion YPF-P could inhibit HSC proliferation significantly,and its mechanism might be related to the inhibition of p-ERK1/2 protein.

关 键 词：肝硬化 血小板源性生长因子 受体 血小板源生长因子 细胞外信号调节MAP激酶类 

分 类 号：R289.5[医药卫生—方剂学] R575.2[医药卫生—中药学]