橙皮苷对非酒精性脂肪肝大鼠COX-2表达及活性的影响  

Effect of hesperidin on the expression and activity of COX-2 in rats with non-alcoholic fatty liver disease

作　　者：王雯[1] 李俊[1] 李荣[1] 熊立[1] 凡小燕[1] 任丹阳[1] 

机构地区：[1]安徽医科大学药学院,安徽天然药物活性研究省级重点实验室,合肥230032

出　　处：《安徽医科大学学报》2011年第9期897-901,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家重大基础研究前期(973前期)研究专项(编号:2002CCC02900)

摘　　要：目的研究橙皮苷(HDN)对大鼠非酒精性脂肪肝(NAFLD)COX-2表达及活性的影响。方法高脂乳剂诱导大鼠NAFLD模型,SD大鼠随机分为正常组、模型组、橙皮苷(80、160、320 mg/kg)组、塞来昔布(10 mg/kg)组和凯西莱(60 mg/kg)组,采用病理组织学方法及对血清总胆固醇(TC)、甘油三酯(TG)水平的检测评价HDN对大鼠NAFLD的治疗作用;采用放射免疫测定法测定血浆中COX-2催化产物6-酮-前列腺素F1α(6-Keto-PGF1α)和血栓素B2(TXB2)的含量;RT-PCR法检测肝组织中COX-2和IL-8的mRNA表达。结果 HDN能改善肝细胞脂肪变性、明显降低大鼠NAFLD血脂TC、TG水平和6-Keto-PGF1α、TXB2的含量,抑制COX-2和IL-8的mRNA表达。结论 HDN对大鼠NAFLD有一定治疗作用,其机制可能与其抑制肝组织中COX-2的表达及活性有关。Objective To investigate the effect of hesperidin（HDN） on the expression and activity of cyclooxyenase-2（COX-2） in non-alcoholic fatty liver disease（NAFLD） rats.Methods The NAFLD rat model was established by giving rats high-fat forage,then the rats were randomly divided into seven groups： normal group,model group,HDN（80,160 and 320 mg/kg） groups,celecoxib（100 mg/kg） group and tripronin（60 mg/kg） group.The treatment of HDN on NAFLD was evaluated by the changes of histopathological and levels of TC and TG in serum.The catalysates of COX-2 were 6-Keto-PGF1α and TXA2 in plasma,which levels were measured by radioimmunoassay.The mRNA expressions of COX-2 and interleukin 8（IL-8） were detected by reverse transcription PCR（RT-PCR）.Results HDN could not only ameliorate hepatocellular steatosis,but also reduce the levels of TC,TG in serum and the levels of 6-Keto-PGF1α,TXB2 in plasma significantly.Furthermore,HDN could inhibit the expressions of COX-2 and IL-8 mRNA in liver tissue.Conclusion HDN has significant therapeutical effect on NAFLD and its possible mechanism is related to inhibiting the activity and expression of COX-2 in liver tissue.

关 键 词：橙皮苷 脂肪肝 环氧化酶2 

分 类 号：R282.71[医药卫生—中药学] R575.5[医药卫生—中医学]