褪黑素与酵母硒联合用药对S180肉瘤细胞的作用及其部分机制  

Effects and partial mechanisms of melatonin in combination with selenium yeast on S180 sarcoma cells

作　　者：王伟迪[1] 徐维平[1,2] 魏伟[1] 路景涛[1] 周冉[1] 黄莺[1] 魏浩洁[1] 

机构地区：[1]安徽医科大学临床药理研究所,抗炎免疫药理学省部共建教育部重点实验室,合肥230032 [2]安徽医科大学附属省立医院,安徽省循证医学中心,合肥230001

出　　处：《安徽医科大学学报》2011年第9期917-922,共6页Acta Universitatis Medicinalis Anhui

基　　金：安徽省人才开发专项基金(编号:2008Z049);安徽省高等学校青年教师科研资助基金(编号:2008jq1068)

摘　　要：目的观察褪黑素(MT)与酵母硒(Se)联合应用对S180肉瘤的治疗效果及其可能机制。方法将S180肉瘤细胞接种于小鼠右腋皮下,将小鼠随机分成7组:模型组、Se(100、200μg/kg)组、MT(40、80 mg/kg)组、MT+Se(40 mg/kg+100μg/kg)组、环磷酰胺(40 mg/kg)组。各组灌胃给予相应药物,模型组给予酵母粉悬浊液。10 d后处死小鼠,剥离肿瘤并称重。比色法测谷胱甘肽过氧化物酶(GPx)活性,DNA电泳及TUNEL观察凋亡,免疫组化法和Western blot法测定肿瘤组织内p53的表达。结果 MT与Se可提高抑瘤率、降低肿瘤重量、提高血清GPx活性、提高p53的表达水平、使凋亡细胞数目增加。与相同剂量的单一用药相比,联合用药的效果更加明显。结论 MT与Se均具有抗肿瘤作用,二者联合应用可提高对肿瘤的治疗效果,其作用机制可能与诱导细胞凋亡有关。Objective To observe the antitumor efficacy of melatonin（MT） combined with selenium（Se） on S180 sarcoma cells and its mechanism.Methods The S180 scrcoma cells were inoculated subcutaneously on the right axilla of male mice which were divided randomly into seven groups： model group,Se（100 μg/kg,200 μg/kg） group,MT（40 mg/kg,80 mg/kg） group,MT＋Se（40 mg/kg,100 μg/kg） group,cyclophosphamide（CTX,40 mg/kg） group.Then,each group was given the corresponding drugs.After ten days,the mice were euthanized.The tumor tissues were examined by immunohistochemistry and Western blot.Glutathione peroxidase（GPx） enzyme activity was detected by colorimetric determination.Results MT and Se were able to improve the tumor inhibition rate,reduce the weight of tumors,improve the GPx enzyme activity,increase the expression of p53 and increase the number of apoptotic cells.Compared with the same dose single agent,the results of MT combined with Se were more obviously.Conclusion MT and Se both had antitumor effect.MT combined with Se could improve the therapeutic effect of tumor and its mechanism might be related to induction of apoptosis.

关 键 词：褪黑激素 硒 药物疗法 联合 细胞凋亡 肉瘤180 肿瘤抑制蛋白质P53 基因 p53 

分 类 号：R347[医药卫生—基础医学] R979.1