丹参酮ⅡA对低分化鼻咽癌细胞氯通道的激活作用  

Activation of chloride channels by tanshinone ⅡA in nasopharyngeal carcinoma cells

作　　者：李媛[1] 刘善文[2] 李华荣[2] 马文波[2] 潘廷才[1] 朱林燕[1] 叶文才[3] 王立伟[2] 陈丽新[1] 

机构地区：[1]暨南大学医学院药理学系,广东广州510632 [2]暨南大学医学院生理学系,广东广州510632 [3]暨南大学药学院,广东广州510632

出　　处：《中国药理学通报》2011年第9期1205-1209,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30771106;30871267;308705672;90913020;U0932004);广东省自然科学基金资助项目(No7005974)

摘　　要：目的研究丹参酮ⅡA对低分化鼻咽癌细胞(CNE-2Z)氯通道的激活作用并分析该通道的生理学和药理学特性。方法采用膜片钳全细胞记录技术记录丹参酮ⅡA激活的CNE-2Z细胞氯电流,细胞外高张刺激或灌流氯离子通道阻断剂,观察并分析电流的特性。结果细胞外灌流1nmol.L-1的丹参酮ⅡA激活CNE-2Z细胞产生一个具有明显外向优势的电流,该电流没有明显的电压依赖性和时间依赖性的失活,电流的翻转电位为(-5.9±0.2)mV,接近氯离子平衡电位。渗透性容积缩小可以完全抑制该电流,氯通道阻断剂NPPB可以部分抑制该电流。结论丹参酮ⅡA激活鼻咽癌细胞膜上的氯通道,诱导氯电流,该电流具有容积敏感性。Aim To study the activation and the prop- erties of chloride channels activated by tanshinone 11 A （Tan 11 A ） in nasopharyngeal carcinoma （CNE-2Z） cells. Methods The whole-cell patch clamp technique was used to record chloride currents. The chloride cur- rents were characterized by extracellular applications of hypertonic challenges and the chloride channel block- ers. Results A chloride current was activated by ex- tracellular applications of tanshinone II A （ 1 nmol · L-1）. The current showed significant outward rectifica- tion and was reversed at a potential （ -5.9 ：l： 0. 2 mV） close to the calculated equilibrium potential for C1-. There was no significant time- or voltage-dependent in-activation. Hypertonicity-induced cell shrinkages or extracellular applications of the chloride channel bloc- ker 5-nitro-2-（ 3-henylpropylamino ） benzoic acid （NPPB） inhibited significantly tanshinone 1/A-activa- ted chloride currents. Conclusions Tanshinone 11 A can induce a C1- current by activation of the chloride channels on the plasmic membrane of CNE-2Z cells. The tanshinone II A-induced current is volume-sensi- tive.

关 键 词：中药药理 丹参酮ⅡA 氯通道 鼻咽肿瘤 氯通道阻 断剂 膜片钳技术 

分 类 号：R284.1[医药卫生—中药学] R329.25[医药卫生—中医学]