脂多糖激活补体诱导内皮细胞释放黏附分子和凋亡  

Adhesion molecule release and apoptosis of endothelial cell induced by lipopolysaccharide-activated complement

作　　者：沈良贤[1,2] 李敏[3] 孙黔云[1] 石京山[2] 

机构地区：[1]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550002 [2]遵义医学院,贵州遵义563000 [3]贵州省人民医院呼吸疾病研究所,贵州贵阳550002

出　　处：《中国药理学通报》2011年第9期1245-1249,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No31060124);国家重点基础研究发展计划(973计划)资助项目(No2009CB526512);贵州省优秀科技教育人才省长专项资金资助项目(No黔省专合字2006-65号);贵州省优秀青年科技人才项目(No黔科合人字2005-0510号)

摘　　要：目的在细胞水平上研究脂多糖(lipopolysaccharide,LPS)激活血清补体对内皮细胞的作用和影响。方法研究LPS激活血清补体的作用方式和特点,观察LPS激活补体对内皮细胞表达黏附分子和凋亡的影响。采用ELISA检测内皮细胞释放P-selectin、E-selectin和ICAM-1的变化,化学发光法检测Caspase-3/7活化情况,SRB法检测LPS激活补体对内皮细胞生长的影响。结果 LPS能够激活血清补体,这种激活呈现量效、时效性。LPS激活血清补体可诱导内皮细胞瞬时明显释放P-selectin,E-selectin和ICAM-1表达也明显上调,同时可导致Caspase-3/7活化。在本实验条件下,LPS激活血清补体对内皮细胞生长未产生抑制。结论 LPS激活血清补体可损伤内皮细胞,导致内皮细胞明显表达黏附分子以及发生凋亡。Aim To investigate the effects of lipopoysaccharide-activated complement on endothelial cells. Methods The manner of LPS activating complement was characterized. Then the effects of LPS-ac- tivated complement on endothelial cells were observed. P-selectin, E-selectin, and ICAM-1 were determined by ELISA. The activity of Caspase-3/7 was measured by chemiluminescence method. The growth of endothelial cells was measured by SRB method. Results LPS activated complement in a doseand time-dependent manner. The incubation mixture of LPS and comple-ment significantly induced the release of P-selectin, E- selectin, and ICAM-1. The activity of Caspase-3/7 was also significantly increased. But the incubation mixture nearly showed no inhibition on the growth of endothelial cells. Conclusions LPS-activated com- plement can injure endothelial cells, and significantly up-regulate the expression of adhesion molecules and apoptosis.

关 键 词：脂多糖 补体 内皮细胞 黏附分子 凋亡 补体激 活 眼镜蛇毒因子 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]