辛伐他汀对内皮细胞NADPH氧化酶表达和活性氧生成的影响  

Effects of simvastatin on mRNA expression of NADPH oxidase and intracellular level of reactive oxygen species in HUVEC

作　　者：王蕾[1] 舒旭[1] 伍世挥[1] 吴和平[1] 舒筱灿[1] 罗海[1] 

机构地区：[1]怀化医学高等专科学校基础医学部,湖南怀化418000

出　　处：《中国药理学通报》2011年第9期1276-1278,共3页Chinese Pharmacological Bulletin

基　　金：湖南省教育厅科研基金资助项目(No07C057)

摘　　要：目的探讨辛伐他汀对内皮细胞NADPH氧化酶表达及活性氧生成的影响。方法以不同浓度辛伐他汀和10-8mol.L-1辛伐他汀不同处理时间培养内皮细胞,用RT-PCR检测NADPH氧化酶各亚单位(NOX4、NOX2、p67phox、p22phox、RAC)表达的变化;用流式细胞仪检测细胞内活性氧的浓度。结果辛伐他汀能下调NOX4、p67phox、p22phoxmRNA表达,同时下调细胞内活性氧浓度。结论辛伐他汀可能通过下调NADPH氧化酶表达,以减少活性氧生成从而达到抗氧化损伤作用。Aim To investigate the effects of simvasta- fin on mRNA expression of NADPH oxidase （NOX4, NOX2, p67phox, p22phox, RAC） and intracellular lev- el of reactive oxygen species （ROS） in HUVEC. Methods mRNA expression of NADPH oxidase in HUVEC was allalyzed by RT-PCR. Intracellular ROS level was de｜ected by flow eytometry. Results mRNAexpression of NOX4, p67phox, p22phox and intraeel- lular ROS level were decreased in HUVEC by simvastatin. Conclusion Simvastatin decreases intracellular ROS level possibly by decreasing expression o/I NOX4, p67phox,p22phox in HUVEC.

关 键 词：NADPH氧化酶 活性氧 辛伐他汀 NOX4 NOX2 p67phox P22PHOX RAC 

分 类 号：R322.123[医药卫生—人体解剖和组织胚胎学] R329.24[医药卫生—基础医学]