解毒化瘀方含药血清对K562/A02细胞凋亡抑制蛋白及核周因子κB表达的影响  

Effect of Jiedu Huayu Recipe Containing Serum on the Expressions of Survivin and NF-κB of K562/A02 Cell Apoptosis by

作　　者：马武开[1] 李玉莹[1] 姚血明[1] 黄礼明[1] 姚宇红[1] 

机构地区：[1]贵阳中医学院第二附属医院血液风湿科,贵阳550003

出　　处：《中国中西医结合杂志》2011年第8期1097-1100,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30860353)

摘　　要：目的探讨解毒化瘀方含药血清对白血病K562/A02细胞凋亡抑制蛋白survivin基因和核周因子κB(NF-κB)信号基因表达的影响。方法将K562/A02细胞分为6个组,分别加入等体积牛血清,兔血清,高、中、低剂量中药血清和干扰素,并设K562敏感细胞株为对照。采用半定量逆转录聚合酶链反应(RT-PCR)法检测解毒化瘀方含药血清处理后K562/A02细胞survivin的表达;Western blot法检测NF-κB/P65、NF-κB/P50、IκBα的表达。结果 survivin耐药基因在K562/A02细胞中高表达,加用解毒化瘀含药血清处理后,高、中剂量中药血清组和干扰素对照组能够survivin的表达明显降低。NF-κB在K562/A02耐药细胞亦呈高表达,加用解毒化瘀含药血清处理后,P65、P50和IκBα的表达均有不同程度下降,以高、中剂量组最明显,且高剂量组P65、P50表达下降优于干扰素对照组(P<0.05),IκBα下降与干扰素对照组相当(P>0.05)。结论解毒化瘀方能够降低K562/A02细胞NF-κB信号基因的表达,影响NF-κB-survivin途径逆转耐药。Objective To study the effect of Jiedu Huayu Recipe containing serum on the expressions of survivin gene of leukemia K562/A02 cell apoptosis and the expression of nuclear factor kappa B （NF-κB）.Methods K562/A02 cells were divided into six groups.An equal volume of calf serum,rabbit serum,high,middle,and low dose Jiedu Huayu Recipe containing serum,and interferon was respectively added.K562 sensitive cell strain was set up as the control.The expressions of survivin in K562/A02 cells after treated with Jiedu Huayu Recipe containing serum were detected using semi-quantitative reverse transcriptase polymerase chain reaction method.The expressions of NF-κB/P65,NF-κB/P50,and IκBα were detected using Western blot method.Results The survivin resistant gene was highly expressed in K562/A02 cells.After treated by Jiedu Huayu Recipe containing serum,the expressions of survivin could be obviously lowered in the high and middle dose Jiedu Huayu Recipe containing serum groups as well as the interferon control group.NF-κB was also highly expressed in K562/A02 resistant cells.After treated by Jiedu Huayu Recipe containing serum,the expressions of P65,P50,and IκBα decreased to some degrees.The decrement was the most obvious in the middle and high dose Jiedu Huayu Recipe containing serum groups.The decreased expressions of P65 and P50 in the high dose Jiedu Huayu Recipe containing serum group were higher than that in the interferon control group （P0.05）.The decrement of IκBα was equivalent to that of the interferon control group （P0.05）.Conclusion Jiedu Huayu Recipe could block the NF-κB signal pathway of K562/A02 cells and reverse drug resistance by influencing the expression of NF-KB-survivin genes.

关 键 词：白血病 多药耐药 K562/A02细胞 survivin 核周因子κB信号 解毒化瘀方 

分 类 号：R285.5[医药卫生—中药学]