光氧化处理脱细胞牛心包构建组织工程心肌补片的实验研究  

Experiment study of the acellular bovine pericardium treated by dye-mediated photooxidation used as engineering heart tissue

作　　者：张振亮[1] 周建业[1] 胡盛寿[1] 刘立群[2] 孙平平 杨子鹤[1] 李君[1] 

机构地区：[1]中国医学科学院阜外心血管病医院卫生部心血管疾病再生医学重点实验室,100037 [2]潍坊医学院附属医院心胸外科 [3]山西省第二医院肾科

出　　处：《中华胸心血管外科杂志》2011年第8期485-488,共4页Chinese Journal of Thoracic and Cardiovascular Surgery

基　　金：本课题受国家自然科学基金资助（30772147）

摘　　要：目的探讨经染料介导光氧化处理的脱细胞牛心包构建组织工程心肌补片的可行性。方法新鲜牛心包先脱细胞，再经光氧化法处理，消毒后种植雄性sD骨髓间充质干细胞（MSCs）。结扎雌性大鼠左冠状动脉前降支，制作心肌梗死模型，1周后将符合心肌梗死标准的大鼠随机分成3组，心肌梗死对照组（MI）、补片组（P）、种细胞补片组（P＋C）分别进行干预。4周后，超声评价心功能；2周、4周取材行组织学和免疫组化检查。结果脱细胞处理完全去除了牛心包组织中的细胞，光氧化处理使组织结构致密；种植的细胞在组织表面形成连续的细胞层。P＋C组牛心包心肌补片降解程度、微血管密度在2周、4周时均较P组大。超声评价补片4周后大鼠心功能，P＋C组左室射血分数（LYEF）、左室缩短分数（LVFS）均较MI组和P组大，与MI组的差异有统计学意义。结论光氧化处理脱细胞牛心包构建的组织工程心肌补片可以延缓心功能恶化，该处理方法具有良好的应用潜力。Objective To evaluate the feasibility of constructing tissue engineering cardiac patch with photooxidationfixed acellular bovine pericardium. Methods Fresh bovine pericardia were treated by dye-mediated photooxidation after decellularization. Some of them were seeded with bone marrow stromal cells（MSCs） isolated from male SD rats to construct cardiac patches. Myocardial infarction（MI） model was made in female SD rats by left anterior descending coronary ligation（LAD）. One week later, the confirmed MI rats were divided into three groups randomly, group MI （ n = 15 ） without any treatment; group P（ n = 18 ）with photooxidated pericardia implantation; group P ＋ C （n = 18 ）with seeded pericardia implantation. A sham group （ n = 10） was also performed with opening and closing chest twice only. The heart were explanted at 2 or 4 weeks after implantation, and examined histologically and immunohistochemically. The heart function was evaluated by echocardiography at 4 weeks before excising the rats. Results There were no cells or cell debris remained in bovine pericardium tissue. The fiber structure became condensed after photooxidation. The seeded cells formed a continuous layer on the surface of the tissue. The pericardial degradation level and newly formed microvessel density were larger in group P ＋ C than in group P after 2[ （ 13.7 ± 5.2）个/mm2 vs （7.1 ＋ 3.1 ） 个/mm2, P 〈 0. 05 ] and 4 [ （ 22.6± 4.9 ）个/mm2 vs （ 14. 1 ± 5.3 ） 个/mm2, P 〈 0.05 ] weeks. Four weeks after transplantation, cardiac echoeardiography showed left ventricular ejection fraction（LYEF）was lower in group MI（44.8 ±4.4） % and group P（48.4 ±5.0） % compared with group P ＋ C（49.3 ±4.8） % , left ventrienlar fractional shorterning（LVFS） was lower in group MI （ 18.0 ±2.2 ） % and group P （ 19.8 ± 2.5 ） % compared with group P ＋ C （ 20.4 ± 2.5 ） % , the difference between P ＋ C and MI was significant. Conclusion Transplantation of the tissue engineered bo

关 键 词：心肌 组织工程 心肌梗塞 牛心包 光氧化 

分 类 号：R542.22[医药卫生—心血管疾病]