网织血小板和血小板相关抗体在特发性血小板减少性紫癜鉴别诊断中的作用比较  

Role of Reticulated Platelets and Platelet-Associated Antibody in Differential Diagnosis of Idiopathic Thrombocytopenic Purpura

作　　者：刘艳[1] 王贞[1] 袁百香[1] 王晓燕[1] 吴琼[1] 袁宏[1] 

机构地区：[1]大连医科大学附属第一医院检验科,辽宁大连116011

出　　处：《中国实验血液学杂志》2011年第4期979-982,共4页Journal of Experimental Hematology

摘　　要：本研究旨在探讨网织血小板和血小板相关抗体在特发性血小板减少性紫癜(ITP)鉴别诊断中的作用。用Sysmex XE-5000全自动血细胞分析仪对34例ITP患者、36例非ITP患者及411例正常人静脉血进行检测,获得未成熟血小板比例(immature platelets fraction,IPF)数据,同时利用酶联免疫法测定血小板相关抗体含量,并对二者结果进行统计学分析。结果表明,ITP患者的IPF值明显高于疾病对照组和正常对照组,差异显著(p均<0.05)。对于ITP诊断的意义,IPF的阳性率明显高于疾病对照组,差异显著(p<0.05);PAIgG的阳性率与疾病对照组比较,无显著性差异(p>0.05)。IPF的灵敏度、特异度、阴性预测值均高于PAIgG,仅阳性预测值略低于PAIgG。IPF的ROC曲线下面积大于PAIgG的ROC曲线下面积。结论IPF和PAIgG对于ITP的鉴别诊断均有一定的临床意义,IPF的诊断价值优于PAIgG。The objective of this study was to evaluate the role of reticulated platelets （RF） and platelet-associated antibody（PAIg） in differential diagnosis of idiopathic thrombocytopenic purpura （ITP）. The immature platelet fraction （IPF） of 34 1TP patients, 36 non-ITP patients and 411 normal people were detected by Sysmex XE-5000 automated blood cell analyser and PAIgG content was detected by enzyme-linked immunosorbent assay. All results were analysed statistically. The results showed that IPF of ITP patients was significantly higher than that of non-ITP patients and normal people respectively（p 〈0.05）. For diagnosis of ITP,IPF positive rate of ITP patients was significantly higher than that of non-ITP patients （p 〈 0.05 ）. There was no significant difference between the positive rate of PAIgG in ITP and non- ITP patients （p 〉 0.05 ）. The sensibility, specificity and negative predictive value of IPF were higher than those of PAIgG, while the positive predictive value of IPF was a little lower than that of PAIgG. The area under receiver operating characteristic curve of IPF was larger than that of PAIgG. It is concluded that both IPF and PAIgG detection are valuable in differential diagnosis of ITP. The diagnostic value of IPF is better than that of PAIgG.

关 键 词：网织血小板 未成熟血小板比例 血小板相关抗体 特发性血小板减少性紫癜 

分 类 号：R558.2[医药卫生—血液循环系统疾病] R554.6[医药卫生—内科学]