全自动微柱玻璃珠技术提高血型相容性检测效能  

Efficiency Improvement of Fully Automatic Microcolumn Glass Sphere Technology Used in Blood Group Compatibility Tests

作　　者：蒋光明[1] 王保龙[1] 完晓菊[1] 王敏[1] 周建华[1] 廖艳秋[1] 

机构地区：[1]安徽省立医院输血科,安徽合肥230001

出　　处：《中国实验血液学杂志》2011年第4期1048-1052,共5页Journal of Experimental Hematology

摘　　要：探讨基于微柱玻璃珠技术的ORTHO AutoVue Innova全自动血型及配血分析系统能否满足输血科血型血清学实验要求。用ORTHO AutoVue Innova全自动血型及配血分析系统和盐水介质法分别检测IgM类抗C、抗c、抗D、抗E和抗e系列稀释液各16份与相应抗原阳性的红细胞凝集反应的强度;用ORTHO AutoVue Innova全自动血型及配血分析系统、凝聚胺法和抗人球蛋白法分别检测IgG类抗D稀释液各16份与RhD阳性红细胞凝集反应的强度;再比较分析微柱玻璃珠法与传统对照实验的检测灵敏度。结果表明,ORTHO AutoVue Innova全自动血型及配血法对IgM类抗C、抗c、抗D、抗E和抗e的检测灵敏度分别为1∶69.8、1∶33.4、1∶1448.1、1∶139.6和1∶32.0;盐水介质法的相应灵敏度分别为1∶16.7、1∶16.6、1∶430.5、1∶34.9和1∶9.9。2种方法检测灵敏度的差异均具有统计学意义(IgM类抗C、抗c、抗D、抗E及抗e的t值分别为14.38、5.48、10.25、12.65和9.59,p均<0.05)。对于IgG类抗D微柱玻璃珠法、凝聚胺法和抗人球蛋白法的检测灵敏度分别为1∶980.6、1∶181.0和1∶304.4,三种检测方法间灵敏度的差异具有统计学意义(F=51.15,p<0.01)。结论:ORTHO AutoVue Innova全自动血型及配血法比传统方法具有更高的检测灵敏度,将其用于常规血型血清学检测是安全可靠的。The purpose of this study was to determine whether the fully automated ORTHO AutoVue Innova system, which based on the microcolumn glass sphere technology, is accurate enough to meet immunohematology testing needs at blood banks. 16 lgM anti-C, anti-c,anti-D,anti-E and anti-e dilution series were tested respectively, with corresponding antigen positive red blood cell solutions, by ORTHO AutoVue Innova system and saline medium test. 16 lgG anti-D dilution series were tested respectively with RhD positive red blood cell solutions by ORTHO AutoVue Innova system, polybrene test and antiglobulin test. The accuracies of microcolumn glass sphere technology were analysed, by comparing to the referenc assays. The results showed that the sensitivities of the ORTHO AutoVue Innova tests were 1： 69.8, 1： 33.4, 1 ： 1448.1, 1 ： 139.6 and 1 ： 32.0 for IgM anti-C, anti-c, anti-D, anti-E and anti-e respectively; the corresponding value of saline medium tests were 1： 16.7,1： 16.6,1 ： 430.5,1 ： 34.9 and 1 ： 9.9. There were statistically significant differences between the groups of each tests （ t values were 14.38, 5.48, 10.25, 12.65 and 9.59 for IgM anti-C, anti-c, anti-D, anti-E and anti-e respectively ,p 〈 0.05 ）. For IgG anti-D,the sensitivities of the ORTHO AutoVue Innova test, polybrene test and antiglobulin test were 1： 980.6,1： 181.0 and 1： 304.4 respectively. There was statistically significant difference among the 3 groups （ F = 51.15 ,p 〈 0.01 ）. It is concluded the use of ORTHO AutoVue Innova system for blood group compatibility test can obtain more accurate results than traditional tube tests, it is reliable and safe for routine tests performed in immunohaematology laboratories.

关 键 词：微柱玻璃珠技术 全自动血型系统 配血分析系统 血型相容性 抗人球蛋白实验 凝聚胺实验 

分 类 号：R446.11[医药卫生—诊断学] R457.1[医药卫生—临床医学]