关节软骨及骨破坏模型大鼠经盐酸氨基葡萄糖干预的最佳剂量  

Minimal effective dose of glucosamine hydrochloride on experimental arthritis in rats

作　　者：王大利 耿成燕 姜丽平 宫德正 仲来福 

机构地区：[1]Department of Laboratory, Dalian Locomotive Hospital, Dalian 116021, Liaoning Province, China [2]China-Japanese Joint Institute for Medical and Pharmaceutical Science, Dalian Medical University, Dalian 116044, Liaoning Province, China

出　　处：《中国组织工程研究与临床康复》2011年第28期5145-5148,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：日本国日中替代医疗食品研究会项目(20050401)资助~~

摘　　要：背景:研究表明盐酸氨基葡萄糖可减轻骨关节炎的症状并能保护关节软骨,但该药的最佳剂量至今尚无共识。目的:观察盐酸氨基葡萄糖对由白陶土与鹿角菜胶诱发大鼠关节炎的治疗最低有效剂量。方法:将SD大鼠分别用蒸馏水,盐酸氨基葡萄糖溶液0.4,0.8及1.6g/kg灌胃,采用白陶土与鹿角菜胶诱发大鼠单发关节炎模型。建模后1,3,5d,用容积测量法测定各组大鼠的左右后肢足跖体积,计算足跖肿胀度。用游标卡尺测定其胫跗骨关节最大径;测定足跖伊文思蓝含量,右足跖制备组织切片,染色后镜下观察其病变程度并进行评分。结果与结论:给予大鼠盐酸氨基葡萄糖1,3和5d后,盐酸氨基葡萄糖0.8及1.6g/kg组大鼠右后足跖肿胀度及胫跗骨关节径明显小于模型组(P<0.05或P<0.01),足跖肿胀度及胫跗骨关节径均有随给药剂量增大而减小。给药5d后,盐酸氨基葡萄糖0.8及1.6g/kg组大鼠右后足跖伊文思蓝含量及组织病理学评分明显小于模型组(P<0.05或P<0.01)。结果提示,盐酸氨基葡萄糖能够有效地预防骨关节炎关节软骨和骨的破坏,并可预防关节周围组织炎症,其最低有效剂量为0.8g/kg。BACKGROUND：Studies have shown that glucosamine hydrochloride （GAH） may lessen the symptoms of osteoarthritis and protect articular cartilage, however, its optimal dose remains unclear. OBJECTIVE：To observe the minimal effective dose of GAH on the kaolin and carrageenan-induced arthritis in rat models. METHODS：Rats in experimental groups received distilled water （model control） or GAH at 0.4, 0.8 and 1.6 g/kg for 1 hour, respectively, twice daily for 5 consecutive days. The arthritis was induced by kaolin and carrageenan 1 hour after administration of GAH. The volume （mL） of hind paws for rats in each group was estimated 1, 3, 5 days after induction of arthritis. The degree of right hind paw swelling was calculated. The maximal diameter of tibio-tarsal articulation was measured with a vernier caliper. The concentration of Evans blue was also determined. The histopathlolgical severity of right hind paw in arthritis modes was evaluated and scored. RESULTS AND CONCLUSION：The degree of hind paw swelling of right hind paw and diameter of tibio-tarsal articulation showed a statistically significant reduction in rats given GAH 0.8 and 1.6 g/kg compared with rats in model control at 1, 3, 5 days （P 0.05 or P 0.01）, exerting a dose-dependent manner. The content of Evans blue and histopathological scores significantly decreased in rats given GAH 0.8 and 1.6 g/kg for 5 days compared with model control group （P 0.05 or P 0.01）. GAH is effective in preventing destruction of cartilage bone and inflammation of soft tissue around articulation in the rat model of kaolin and carrageenan-induced arthritis, and the minimal effective dose is 0.8 g/kg.

关 键 词：盐酸氨基葡萄糖 白陶土 鹿角菜胶 骨关节炎 大鼠 骨组织工程 

分 类 号：R969[医药卫生—药理学]