转腺病毒-人骨形态发生蛋白7软骨细胞分泌的透明质酸和Ⅱ型胶原  

Secretion of type Ⅱ collagen and hyaluronic acid in chondrocytes transfected by adenovirus-bone morphogenetic protein 7

作　　者：张洁 刘巍 朱新辉 周怡 

机构地区：[1]Department of Immunology, Medical School of Nantong University, Nantong 226001, Jiangsu Province, China [2]Department of Joint Surgery, Second Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China

出　　处：《中国组织工程研究与临床康复》2011年第28期5153-5156,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：南通市社会发展科技计划资助项目(S2008042);江苏省卫生厅开放课题资助项目(KF200953);江苏高校优势学科建设工程资助项目~~

摘　　要：背景:软骨细胞自身分裂能力不强和去分化现象限制了其在组织工程中的应用。目的:观察腺病毒-骨形态发生蛋白7转染兔软骨细胞后骨形态发生蛋白7的表达及其对软骨细胞分泌Ⅱ型胶原和透明质酸功能的影响。方法:包装骨形态发生蛋白7腺病毒载体,将其转染至兔第2代软骨细胞。检测骨形态发生蛋白7mRNA及蛋白的表达;检测软骨细胞中Ⅱ型胶原和透明质酸的变化。结果与结论:转染腺病毒-骨形态发生蛋白7后48,72h,RT-PCR和Western blot方法显示软骨细胞表达的骨形态发生蛋白7mRNA和蛋白均明显增加,RT-PCR和ELISA显示软骨细胞分泌的Ⅱ型胶原和透明质酸也显著增加。说明应用腺病毒可成功将骨形态发生蛋白7转染至兔软骨细胞,并能促进软骨细胞分泌Ⅱ型胶原和透明质酸。BACKGROUND：Chondrocytes are limited in tissue engineering due to their poor self-dividing capacity and defifferentiation. OBJECTIVE：To investigate the expression of bone morphogenetic protein 7 （BMP-7） in chondrocytes transfected by adenovirus BMP-7 and the effects of transfection on chondrocyte secretion of typeⅡcollagen and hyaluronic acid. METHODS：Adenoviral vector containing BMP-7 was prepared and then transfected into rabbit passage chondrocytes. BMP-7 mRNA and protein expressions in chondrocytes were detected. Changes in type Ⅱ collagen and hyaluronic acid in chondrocytes were determined.RESULTS AND CONCLUSION：At 48 and 72 hours after transfection, RT-PCR and western blot analysis showed that BMP-7 mRNA and protein expressions in the chondrocytes were increased;RT-PCR and ELISA showed the type Ⅱ collagen and hyaluronic acid in the chondrocytes were also obviously increased. These findings suggest that BMP-7 can be successfully transfected into chondrocytes by adenovirus and promote the secretion of type Ⅱcollagen and hyaluronic acid.

关 键 词：骨形态发生蛋白7 软骨细胞 基因转染 Ⅱ型胶原 透明质酸 软骨组织工程 

分 类 号：R681.53[医药卫生—骨科学]