肌肉生长抑制素基因多态性与肌肉生长敏感性的关联  

Association of myostatin polymorphism with sensitivity to muscular growth

作　　者：李晓[1] 刘丽红[2] 马力宏[3] 

机构地区：[1]焦作师范高等专科学校体育系,河南省氐作市454152 [2]大连医科大学基础医学院,辽宁省大连市116044 [3]天津体育学院运动人体科学系,天津市300381

出　　处：《中国组织工程研究与临床康复》2011年第28期5262-5264,共3页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:肌肉生长抑制素(growth differentiation factor 8,GDF-8)基因在人类中对于调节肌肉生长起重要的作用。但关于中国人群该基因的研究甚少。目的:实验从肌肉生长抑制素基因多性AluⅠ酶切位点限制性酶切位点入手,观察GDF-8基因多性与人体肌肉生长的关联。方法:选取天津体育学院无训练汉族学生92名,进行为期2个月的力量训练,用B超测定运动前后肱二头肌和股四头肌的肌肉厚度。测试训练前后形态学各指标包括身高、体质量、体脂肪含量、瘦体质量的变化、运动前后肱二头肌和股四头肌的肌肉厚度变化趋势以及在GDF-8AluⅠ酶切位点限制性酶切位点上的差异。含AluⅠ位点片段由引物扩增后长度为135bp,定义为A,经AluⅠ限制性酶切割后,含有AluⅠ酶切位点的等位基因出现80,55bp的片段,定义为T,这样即出现3种基因型AA,AT和TT。结果与结论:从形态学指标上受试者群体在AluⅠ位点上A/T杂合子及T/T纯合子在身高,训练前后的体质量,瘦体质量,肌肉厚度和肱二头肌及股四头肌直径厚度上均高于A/A纯合子(P<0.05或P<0.01)。结果证实,AluⅠ位点多态性与人体身高,体质量及瘦体质量关联,等位基因T为肌肉生长的先天敏感因子。BACKGROUND：The myostatin plays an important role in the regulation of muscular growth in human body, however, there is few study regarding this gene in Chinese populations. OBJECTIVE：To elucidate the association of growth differentiation factor 8 （GDF-8） gene polymorphism and human muscles through the observation of the Alu I restriction site of GDF-8 gene polymorphism. METHODS：A total of 92 undisciplined male students, of Han population, received strength training for 8 weeks at Tianjin University of Sport, three days a week. The thickness of the biceps and quadriceps was measured with the ultrasonic B test before and after exercise. The body fat, lean body weight, body weight and height were also measured before and after the exercise, as wellas the difference of Alu I restriction site of GDF-8 gene polymorphism. The length of fragments containing Alu I site was 135 bp after primer amplification, and defined as A. after Alu I restriction enzyme digestion, the allele containing Alu I site appeared fragments at 80 and 55 bp, defining as T. Thus three genotypes emerged, namely AA, AT and TT. RESULTS AND CONCLUSION：From the morphologic index, the height, weight, lean body weight, muscle thickness, the biceps and quadriceps muscle diameter of the males at Alu I site A/T heterozygote and T/T homozygote were significantly high than those at A/A homozygote （P 0.05 or 0.01）. The Alu I polymorphism at GDF-8 gene loci is associated with the weight, height and lean body weight. The allele T is the congenital sensitive factor for the muscular growth.

关 键 词：肌肉肥大 肌肉生长抑制素 力量训练 基因多态性 关联研究 

分 类 号：R318[医药卫生—生物医学工程]