护骨素基因T950C及A163G多态性与老年2型糖尿病患者的骨密度  

Association between T950C and A163G polymorphism of the osteoprotegerin gene and bone mineral density in elderly type 2 diabetic patients

作　　者：郏蓉[1] 苗懿德[1] 纪立农[2] 

机构地区：[1]北京大学人民医院老年医学科,北京市100044 [2]北京大学人民医院内分泌科,北京市100044

出　　处：《中国组织工程研究与临床康复》2011年第28期5290-5294,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：北京大学人民医院研究与发展基金交叉课题(RDI2008-02);课题名称:多学科联合进行骨质疏松研究~~

摘　　要：背景:2型糖尿病患者发生骨质疏松症的比率较高。目的:观察老年2型糖尿病患者护骨素基因启动子区域T950C、A163G位点多态性与骨密度的关系。方法:纳入147例老年2型糖尿病患者,男性100例,女性47例,应用多聚酶链反应-限制性片段长度多态性方法测定患者护骨素基因T950C、A163G的基因型;采用双能X线骨密度吸收仪测定患者腰椎、髋部及前臂的骨密度。结果与结论:在老年女性2型糖尿病患者中,T950C不同基因型在特定部位具不同骨密度,CC基因型的腰椎L2、L4骨密度高于TC或TT型;在老年男性2型糖尿病患者中,未发现T950C不同基因型与骨密度相关。在老年女性2型糖尿病患者中,A163G不同基因型在特定部位具不同骨密度,AA型的股骨大转子、前臂骨密度高于AG或GG型;在老年男性2型糖尿病患者中AA型的腰椎L3、L4骨密度高于AG或GG型。表明护骨素基因启动子区T950C基因多态性与老年女性2型糖尿病患者的骨密度相关,A163G基因多态性与老年男、女性2型糖尿病患者的骨密度皆相关。BACKGROUND：Patients with type 2 diabetes mellitus are in high risk with osteoporosis, but the pathogenesis is not clear. OBJECTIVE： To investigate the association between T950C and A163G polymorphisms of the osteoprotegerin （OPG） gene and bone mineral density in the elderly type 2 diabetic patients. METHODS：We determined T950C and A163G polymorphisms of OPG gene by polymerase chain reaction-restriction fragment length polymorphism in 147 elderly type 2 diabetic patients including 100 males and 47 females. Bone mineral density （BMD） at lumbar spine, hip and forearm was assessed by dual-energy X-ray absorptiometry. RESULTS AND CONCLUSION：In elderly female patients with type 2 diabetes mellitus, the CC genotype of the T950C was associated with significantly higher BMD at lumbar spine;while in elderly male patients with type 2 diabetes mellitus, no association was found between the T950C polymorphisms and BMD. In elderly female patients with type 2 diabetes mellitus, the AA genotype of the A163G was associated with significantly higher BMD at femoral torch and forearm area;while in elderly male patients with type 2 diabetes mellitus, the AA genotype of the A163G was associated with significantly higher BMD at lumbar spine. T950C genotypes in the OPG gene promoter were associated with BMD in elderly female patients with type 2 diabetes mellitus. A163G genotypes in the OPG gene promoter were associated with BMD in elderly female and male patients with type 2 diabetes mellitus.

关 键 词：护骨素 基因多态性 2型糖尿病 骨密度 骨质疏松症 

分 类 号：R318[医药卫生—生物医学工程]